Optimized inorganic carbon regime for enhanced growth and lipid accumulation in Chlorella vulgaris

Table 2 FAME profiles of C. vulgaris when supplemented with various bicarbonate salts just prior to nitrogen depletion

Treatment	C16:0	C16:1	C18:0	C18:1	C18:2	C18:3	Other^a	Total biodiesel potential (%)^b	Potential biodiesel productivity (g L⁻¹ day⁻¹)^b
0 mM HCO₃ ⁻	7.51 ± 0.69	1.59 ± 0.16	4.72 ± 0.5	16.82 ± 2.97	3.92 ± 0.43	9.3 ± 0.75	0.4 ± 0.1	44.19 ± 5.42	0.029 ± 0.004
50 mM ACS grade NaHCO₃	7.78 ± 0.46	2.33 ± 0.18	2.46 ± 0.14	31.82 ± 1.73	5.61 ± 0.35	9.06 ± 0.43	0.58 ± 0.06	59.63 ± 3.34^*	0.061 ± 0.008^*
50 mM industrial grade NaHCO₃	7.38 ± 0.37	2.11 ± 0.05	2.28 ± 0.18	30.06 ± 1.39	5.16 ± 0.13	8.43 ± 0.74	0.61 ± 0.05	56.04 ± 2.9^*	0.058 ± 0.001^*
50 mM KHCO₃	6.8 ± 0.22	1.79 ± 0.05	2.64 ± 1.11	27 ± 2.08	5.15 ± 0.07	7.74 ± 0.3	0.54 ± 0.13	51.62 ± 2.25^*	0.048 ± 0.012^*
50 mM Na₂CO₃	5.12 ± 0.11	1.26 ± 0.03	2.56 ± 0.04	14.47 ± 0.12	3.4 ± 0.02	5.84 ± 0.05	0.2 ± 0.05	32.82 ± 0.28	0.02 ± 0.001
25 mM NaHCO₃∙Na₂CO₃	6.69 ± 0.08	1.84 ± 0.03	3.82 ± 0.09	23 ± 0.62	4.67 ± 0.09	7.72 ± 0.12	0.47 ± 0.08	53.33 ± 1.35^*	0.041 ± 0.003^*

Values are reported for the completion of the experiment (5.75 days) (n = 3). All values expressed as weight percent (% weight FAME/weight biomass) unless indicated otherwise
^* p value <0.05 as determined by a two-tailed t test, statistically significant higher [difference between treatment and control group (0 mM HCO₃ ⁻)]
^aSum of other compounds detected
^bTotal FAMEs

ISSN: 2731-3654