Skip to main content

Table 4 C. vulgaris lipid characteristics for cultures grown under various inorganic carbon regimes

From: Optimized inorganic carbon regime for enhanced growth and lipid accumulation in Chlorella vulgaris

Treatment C16:0a C16:1a C18:0a C18:1a C18:2a C18:3a Otherb Total biodiesel potential (%)c Potential biodiesel productivity (g L−1 day−1)c
Optimized Scenario 10.66 ± 0.3 2.76 ± 0.03 5.17 ± 0.09 24.42 ± 0.44 7.17 ± 0.14 10.21 ± 0.33 0.64 ± 0.06 61.04 ± 1.31 0.098 ± 0.002
50 mM NaHCO3∙Na2CO3 9.27 ± 0.34 2.64 ± 0.1 3.97 ± 0.11 19.85 ± 1.2 6.56 ± 0.18 8.45 ± 0.15 0.68 ± 0.09 51.41 ± 2.14* 0.07 ± 0.005*
5 % (v/v) CO2 Continuous 9.45 ± 0.7 1.85 ± 0.14 2.36 ± 0.2 26.59 ± 2.1 4.58 ± 0.4 7.88 ± 0.76 0.63 ± 0.12 53.33 ± 1.35* 0.091 ± 0.005*
  1. Experimental conditions: (1) the optimized scenario of a continuous sparge of atmospheric air supplemented periodically with 5 % CO2 (v/v) to maintain pH between 8.4 and 8.7 and an initial addition of 5 mM NaHCO3 at inoculation plus an additional 50 mM of ACS grade NaHCO3 just prior to nitrogen depletion to stimulate TAG accumulation. (2) The same culture conditions as scenario 1 listed above, except TAG accumulation was induced by adding 25 mM NaHCO3∙Na2CO3 (sesquicarbonate). (3) Continuous sparge of atmospheric air supplemented with 5 % CO2 (v/v) during daytime hours (n = 3). Biodiesel productivity is calculated for the stationary growth phase (e.g., from depletion of nitrogen until termination of experiment)
  2. * p value <0.05 as determined by a two-tailed t test, statistically significantly lower (difference between treatment and optimized scenario)
  3. aAll values expressed as weight percent (% weight FAME/weight biomass)
  4. bSum of other compounds detected
  5. cTotal FAMEs