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Fig. 2 | Biotechnology for Biofuels

Fig. 2

From: Construction of a self-cloning system in the unicellular green alga Pseudochoricystis ellipsoidea

Fig. 2

PCR amplification of the pUT1 sequences integrated into the genomes of transgenic strains. a The pUT1 map: TUBP, the promoter region of PeTUBULIN1; cPeUMPS, the PeUMPS cDNA; ACTT, the terminator region of PeACT1. The positions of the five PCR primers and the PCR products amplified using the primers (1–5) are shown below the pUT1 map. b Agarose gel electrophoresis to verify the presence or absence of PCR products 1–5. M, DNA marker (λ-EcoT14 I digest); P, pUT1; A, 4 M-1A; B, 4 M-1B; C, 4 M-1C; D, 4 M-1D

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