Fig. 4
From: Construction of a self-cloning system in the unicellular green alga Pseudochoricystis ellipsoidea
PCR amplification of the pUT2 sequence that had integrated into the genomes of transgenic strains. a The pUT2 map: RBCSP, the promoter region of PeRBCS; cPeUMPS, PeUMPS cDNA; RBCST, the terminator region of PeRBCS. The positions of the four PCR primers and PCR products amplified using the primers (1–4) are shown below the pUT2 map. b Agarose gel electrophoresis to verify the presence or absence of PCR products 1–4. M, DNA marker (λ-EcoT14 I digest); P, pUT2; A, 4 M-2A; B, 4 M-2B; C, 4 M-2C; D, 4 M-2D; E, 4 M-2E