5-Hydroxymethylfurfural (5-HMF) concentrations relative to growth in C. bescii cultures. 5-HMF (15 mM) was added to LOD medium 4 h after inoculation and residual concentrations were determined via HPLC. a Wild-type, b JWCB018, c JWCB044. Closed circles represent the OD600, open circles represent measured 5-HMF concentrations. For JWCB044 cultures, the 5-HMF concentrations were below detectable limits at t = 48 h and t = 72 h.