Fig. 2

Phylogenetic tree of the structurally characterized family three glycosyl hydrolases. Multiple-sequence alignment was performed using CLUSTALW (http://www.genome.jp/tools/clustalw/), and a phylogenetic tree was constructed using MEGA5 (http://www.megasoftware.net/). Sequences are: Bacillus subtilus Bgl (PDB accession number 4GYJ_A); Streptomyces venezuelae Bgl (PDB accession number 4I3G_A); Kluyveromyces marxianus Bgl (PDB accession number 3AC0_A); Aspergillus aculeatus Bgl (PDB accession number 4IIB_A); Thermotoga neapolitana Bgl (PDB accession number 2X42_A); Trichoderma reesei Bgl (PDB accession number 4I8D_A); and include the 6 putative GH3 sequences from Yarrowia lipolytica genome.