Fig. 3From: Expression of the Acidothermus cellulolyticus E1 endoglucanase in Caldicellulosiruptor bescii enhances its ability to deconstruct crystalline celluloseConfirmation of E1 expression and secretion in C. bescii using Western blot analysis. Concentrated extracellular proteins (10 µg) were electrophoresed in a 15 % gradient Mini-Protean TGX gel (BIO-RAD) and electro-transferred to a PVDF membrane (ImmobilonTM-P; Millipore). The membrane was then probed with an E1 monoclonal antibody. Truncated version of E1 produced in Streptomyces lividans (lane 1); parent strain JWCB018 ΔpyrFA ldh::ISCbe4 Δcbe1 grown at 75 °C (lane 2); E1 expression strain JWCB052 ΔpyrFA ldh::ISCbe4 Δcbe1::P S-layer acel0614 (E1) grown at 75 °C (lane 3), 70 °C (lane 4), and 65 °C (lane 5); MW, MagicMark™ molecular weight marker (Invitrogen).Back to article page