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Fig. 1 | Biotechnology for Biofuels

Fig. 1

From: Deletion of homologs of the SREBP pathway results in hyper-production of cellulases in Neurospora crassa and Trichoderma reesei

Fig. 1

Secretion phenotypes are cellulose-specific. a Total secreted protein as measured by Bradford assay. b SDS-PAGE analysis of total secreted protein, equal volumes loaded. c, d Endoglucanase and xylanase activities, respectively, as measured using dye-release assays. Conidia from wild type and the deletions strains were inoculated directly into growth media containing sucrose (24 h), Avicel (96 h) or xylan (48 h). Protein and enzyme activity levels in the culture supernatants relative to the parental wild-type strain under each condition were analyzed by one-way ANOVA using the Dunnett’s multiple comparisons test. Data drawn from biological replicates (n = 4); error bars indicate standard deviation.

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