Fig. 6

Influence of Xpp1 on xylose reductase expression in T. reesei. T. reesei QM6aΔtmus53 (blue) and the xpp1 deletion strain (green) were grown in MA medium containing 1% (w/v) xylan. Samples were taken after 18, 30, 36, 48, and 72 h growth. a Xylose reductase activity in cell-free extracts was measured in vitro and normalized to the total protein concentration of the cell-free extracts. b Transcript levels of the xyl1 gene were measured by qPCR using sar1 and act transcript levels for normalization and were referred to the reference sample (T. reesei QM6aΔtmus53, 18 h). Results are given as relative transcript ratios in logarithmic scale (Log). The values provided in the figures are means from three biological experiments. Error bars indicate standard deviations.