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Table 1 Activity of wild-type and engineered forms of Cel9A on soluble and microcrystalline cellulose

From: Combining free and aggregated cellulolytic systems in the cellulosome-producing bacterium Ruminiclostridium cellulolyticum

Enzyme

CMC

Avicel

Cel9A

1214 ± 44a,b

236 ± 1.6b,c

Cel9Ac

1284 ± 12

177 ± 1.4

Cel9At

1387 ± 54

164 ± 0.4

  1. Assays were performed at 37°C with 1% (w/v) and 0.35% (w/v) of substrate for CMC and Avicel, respectively.
  2. aValues are µmol of product released per µmol of enzyme min−1.
  3. bAverage and standard deviation of two independent experiments.
  4. cValues are µmol of released products after 24 h of incubation with 0.1 µmol of enzyme.