Fig. 3

Hypersecretion of lignocellulases by Neurospora crassa due to deletion of Ncap3m. Typical secretomes of the wild type (WT) and ΔNcap3m are shown on a sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gel (a, c) after 7 days of culturing in Avicel (a, b) and xylan (c, d) media. Total extracellular protein concentration and enzyme activity (b, d) were measured and evaluated after normalization to the WT control according to percentage (standard error of the mean, n = 3). Asterisks indicate significant differences from the WT (**P < 0.01; ***P < 0.001) based on one-way analysis of variance.