Fig. 4

Restoration of the ΔNcap3m hypersecretion phenotype to normal wild-type (WT) levels by Ncap3m or Trap3m. The following strains were grown in 2% (w/v) Avicel (a) or xylan (b) media: the WT, the Ncap3m gene knockout (KO) mutant (ΔNcap3m), the Ncap3m-complemented strain under either the control of the ccg-1 strong promoter (Pc-Ncap3m) or the native promoter (Pn-Ncap3m), and a Trap3m-complemented strain introduced into ΔNcap3m under the control of the ccg-1 strong promoter (Pc-Trap3m). After 7 days of culturing, the total extracellular protein concentration and endoglucanase (a) or endoxylanase (b) activity were measured. Data were normalized to the WT control according to percentage (standard error of the mean, n = 3); asterisks indicate significant differences from the WT (**P < 0.01; ***P < 0.001; ns not significant) based on one-way analysis of variance.