Fig. 6

Involvement of the adaptor protein 3 (AP-3) complex in lignocellulase secretion in Neurospora crassa. Conidia from the wild type (WT) and Ncap3m and Ncap3b single (ΔNcap3m, ΔNcap3b) and double mutants (ΔNcap3mΔNcap3b) were separately inoculated into Avicel medium and batch cultured for 7 days. The typical secretome of each strain was then observed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) (b), while total extracellular protein concentration and enzyme activity (a) were measured and displayed after normalization to the WT control according to percentage (standard error of the mean, n = 3). Asterisks indicate significant differences from the WT (*P < 0.05; **P < 0.01; ***P < 0.001) based on one-way analysis of variance.