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Table 1 List of extracellular proteins produced by Neurospora crassa mutants for genes orthologous to mutation-hit targets in Trichoderma reesei RUTC30

From: Involvement of the adaptor protein 3 complex in lignocellulase secretion in Neurospora crassa revealed by comparative genomic screening

No.

Mutant (FGSC)

Orthologs in N. crassa a

RUT C30 targetsb

Gene product names

Protein conc. (mg/L)

Increased vs WT (%)c

1

11701

NCU03998

jgi|Trire2|53811|

Adaptor protein complex 3 Mu3A

821.38 ± 21.83

42.9

2

17965

NCU07880

jgi|Trire2|124172|

Protein kinase

780.01 ± 32.87

35.64

3

19384

NCU01161

jgi|Trire2|68926|

Actin polymerization protein Bzz1

773.06 ± 31.51

34.44

4

19393

NCU07492

jgi|Trire2|122689|

Hypothetical protein

758.58 ± 21.01

32.21

5

15743

NCU01242

jgi|Trire2|69437|

G2/mitotic-specific cyclin

759.76 ± 41.93

32.06

6

10372

NCU08807

jgi|Trire2|120117|

cre-1

755.26 ± 51.79

31.22

7

11964

NCU09120

jgi|Trire2|76515|

Lysine-specific histone demethylase Aof2

750.7 ± 20.09

30.6

8

15360

NCU06992

jgi|Trire2|31118|

DNA repair protein Nse1

737.31 ± 37.42

28.59

9

16681

NCU04201

jgi|Trire2|106234|

Signal transduction protein Syg1

729.66 ± 38.29

26.84

10

12039

NCU08441

jgi|Trire2|123786|

Non-ribosomal peptide synthetase

729.27 ± 112.17

26.36

11

12276

NCU03145

jgi|Trire2|78301|

Vacuolar membrane zinc transporter

723.79 ± 40.24

25.81

12

13177

NCU01838

jgi|Trire2|65106|

Nitrilase

722.38 ± 13.8

25.71

13

11468

NCU04142

jgi|Trire2|123114|

Heat shock protein 80

717.96 ± 59.51

24.68

14

12062

NCU07498

jgi|Trire2|79304|

DNA excision repair protein Rad2

715.8 ± 57.68

24.32

15

16319

NCU02842

jgi|Trire2|121087|

Hypothetical protein

712.55 ± 32.87

23.89

16

18830

NCU03811

jgi|Trire2|121453|

Hypothetical protein

710.24 ± 91.52

23.16

17

19831

NCU03545

jgi|Trire2|82153|

Hypothetical protein

706.55 ± 32.91

22.85

18

11956

NCU08809

jgi|Trire2|74765|

Hypothetical protein

704.12 ± 19.44

22.5

19

16594

NCU03914

jgi|Trire2|64375|

Glucan 1,3-beta-glucosidase

704.88 ± 64.43

22.38

20

20296

NCU08364

jgi|Trire2|78320|

Choline sulfatase

702.59 ± 19.06

22.23

21

11040

NCU05411

jgi|Trire2|70071|

Pathway-specific nitrogen regulator

701.46 ± 65.9

21.78

22

19539

NCU07334

jgi|Trire2|68425|

Uracil permease

698.2 ± 25.84

21.72

23

18971

NCU00503

jgi|Trire2|71037|

Nonselective cation channel protein

700.38 ± 66.13

21.59

24

13289

NCU00427

jgi|Trire2|80691|

Hypothetical protein

698.5 ± 19.9

21.52

25

15939

NCU00754

jgi|Trire2|58561|

Multidrug resistant protein

697.71 ± 79.14

21.05

26

13436

NCU05089

jgi|Trire2|64882|

MFS monocarboxylate transporter

692.03 ± 30.99

20.33

27

16570

NCU04886

jgi|Trire2|28409|

MFS multidrug transporter

693.08 ± 69.06

20.3

28

16331

NCU03068

jgi|Trire2|58790|

Glycerol-3-phosphate phosphatase 1

690.98 ± 32.01

20.14

29

20407

NCU11050

jgi|Trire2|75105|

DUF455 domain-containing protein

689.63 ± 40.55

19.86

30

11084

NCU09549

jgi|Trire2|26255|

C6 zinc finger domain-containing protein

683.56 ± 79.47

18.58

31

18230

NCU09887

jgi|Trire2|67030|

Drp1p

678.63 ± 42.93

17.93

32

19296

NCU07276

jgi|Trire2|67732|

ABC bile acid transporter

677.02 ± 69.64

17.5

33

15452

NCU06309

jgi|Trire2|22841|

Hypothetical protein

674.61 ± 18.59

17.36

34

15930

NCU00648

jgi|Trire2|59952|

Choline transporter

675.67 ± 114.38

17.02

35

13757

NCU01420

jgi|Trire2|54157|

Hypothetical protein

672.9 ± 36.3

16.97

36

15627

NCU04626

jgi|Trire2|28731|

G-protein coupled receptor

667.67 ± 37.79

16.05

37

15627

NCU04626

jgi|Trire2|123806|

G-protein coupled receptor

667.67 ± 37.79

16.05

38

16748

NCU00799

jgi|Trire2|40758|

Homocysteine S-methyltransferase

669.17 ± 128.93

15.8

39

14141

NCU08499

jgi|Trire2|58161|

GTPase-activating protein GYP5

663.7 ± 13.31

15.49

40

13568

NCU04521

jgi|Trire2|54511|

Hypothetical protein

665.59 ± 127.62

15.19

41

20195

NCU06578

jgi|Trire2|104161|

KapG

662.02 ± 104.79

14.69

42

19233

NCU01997

jgi|Trire2|74570|

ABC transporter

662.83 ± 141.67

14.63

43

12060

NCU07381

jgi|Trire2|3027|

DNA cross-link repair protein pso2/snm1

659.15 ± 93.95

14.25

44

18123

NCU07703

jgi|Trire2|55887|

Hypothetical protein

655.25 ± 49.89

13.82

45

13910

NCU05195

jgi|Trire2|75072|

Hypothetical protein

653.76 ± 52.14

13.55

46

11279

NCU03125

jgi|Trire2|79405|

NIMA-interacting protein TinC

651.64 ± 26.12

13.32

47

12569

NCU00321

jgi|Trire2|67024|

Hypothetical protein

652.72 ± 75.61

13.23

48

19403

NCU07564

jgi|Trire2|78465|

Siderophore iron transporter mirC

648.04 ± 5.97

12.87

49

15566

NCU01961

jgi|Trire2|59147|

DNA lyase Apn2

647.69 ± 69.51

12.39

50

19712

NCU00497

jgi|Trire2|58391|

Hypothetical protein

643.14 ± 129.41

11.27

51

11150

NCU00278

jgi|Trire2|73912|

Hypothetical protein

643.22 ± 144.06

11.2

52

11459

NCU00340

jgi|Trire2|36543|

Transcription factor steA

637.2 ± 73.32

10.54

53

13984

NCU05837

jgi|Trire2|65104|

Vacuolar protein sorting-associated protein 13a

635.14 ± 16.93

10.5

54

19183

NCU05477

jgi|Trire2|102776|

Hypothetical protein

632.43 ± 18.1

10.02

55

11061

NCU08443

jgi|Trire2|77513|

Transcription factor ace3

635.36 ± 137.21

9.87

56

13086

NCU00541

jgi|Trire2|80592|

Hypothetical protein

627.2 ± 73.28

8.8

57

16803

NCU04809

jgi|Trire2|82037|

MFS phospholipid transporter

630.47 ± 248.43

8.4

58

15478

NCU08642

jgi|Trire2|78268|

Cyclic nucleotide-binding domain-containing protein

624.52 ± 83.42

8.28

59

15647

NCU00025

jgi|Trire2|82499|

Integral membrane protein

623.56 ± 73.21

8.17

60

16280

NCU04108

jgi|Trire2|105874|

Isoamyl alcohol oxidase

616.8 ± 25.92

7.26

61

15869

NCU00335

jgi|Trire2|5140|

Pre-mRNA-splicing factor cwc15

612.18 ± 15.31

6.51

62

13160

NCU01633

jgi|Trire2|62380|

Hexose transporter HXT13

611.29 ± 19.85

6.33

63

19733

NCU06832

jgi|Trire2|112231|

Kinesin

609.8 ± 45.91

5.93

64

12341

NCU09864

jgi|Trire2|56726|

2-Oxoisovalerate dehydrogenase alpha subunit

608.18 ± 4.15

5.88

65

11030

NCU07788

jgi|Trire2|52368|

Fungal specific transcription factor

606.86 ± 36.13

5.47

66

12282

NCU06647

jgi|Trire2|5403|

Enoyl-CoA hydratase/isomerase

605.74 ± 7.76

5.43

67

12018

NCU02751

jgi|Trire2|120806|

Serine/threonine-protein kinase

605.22 ± 59.32

5.05

68

11677

NCU01868

jgi|Trire2|59388|

MFS maltose permease MalP

595.74 ± 3.43

3.71

69

18917

NCU05459

jgi|Trire2|65773|

Mitochondrial AAA ATPase

595.8 ± 64.3

3.39

70

19405

NCU07574

jgi|Trire2|22294|

Hypothetical protein

591.48 ± 5.41

2.96

71

17946

NCU04755

jgi|Trire2|45456|

Protein kinase domain-containing protein ppk32

581.14 ± 12.76

1.12

72

17081

NCU01044

jgi|Trire2|63464|

Hypothetical protein

580.08 ± 10.41

0.95

73

19059

NCU08307

jgi|Trire2|56077|

Hypothetical protein

579.21 ± 100.55

0.3

74

17389

NCU02337

jgi|Trire2|80332|

Mitochondrial carrier protein

576.12 ± 32.03

0.14

75

2489

#####

#####

Wild type

575.45 ± 36.81

0

76

16836

NCU04847

jgi|Trire2|52520|

cyclin

573.97 ± 15.22

−0.13

77

20073

NCU06341

jgi|Trire2|44956|

MFS transporter

573.58 ± 9.57

−0.17

78

19245

NCU02220

jgi|Trire2|64866|

hypothetical protein

563.98 ± 19.59

−1.9

79

12078

NCU00523

jgi|Trire2|50268|

NAD-dependent deacetylase sirtuin-2

551.74 ± 23.25

−4.05

80

12072

NCU04203

jgi|Trire2|121351|

Glucosidase II alpha subunit

508.03 ± 35.04

−11.73

81

13475

NCU07119

jgi|Trire2|60458|

Nonribosomal peptide synthase 2

503.65 ± 12.98

−12.37

82

19165

NCU05213

jgi|Trire2|75074|

Hypothetical protein

449.4 ± 86.39

−22.22

83

18185

NCU08452

jgi|Trire2|110570|

Hypothetical protein

439.95 ± 23.92

−23.52

84

16956

NCU02152

jgi|Trire2|3400|

RRM domain-containing protein

430.62 ± 18.69

25.11

85

11357

NCU03184

jgi|Trire2|4921|

C2H2 conidiation transcription factor FlbC

419.08 ± 52.77

27.31

86

20306

NCU08380

jgi|Trire2|122050|

Plasma membrane phosphatase

416.5 ± 18.07

27.57

87

11360

NCU03244

jgi|Trire2|62053|

WD repeat protein

346.47 ± 27.22

39.81

  1. a N. crassa orthologs: locus selected according to the N. crassa database (v7) (https://www.broadinstitute.org/annotation/genome/neurospora/MultiHome.html).
  2. bRUTC30 targets: locus selected according to the T. reesei genome sequence web site (http://genome.jgi-psf.org/Trire2/Trire2.home.html).
  3. cThe increased percentage of secreted protein relative to the WT. Strains more than 25% increased or decreased compared with the WT are shown in italics.