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Table 1 List of extracellular proteins produced by Neurospora crassa mutants for genes orthologous to mutation-hit targets in Trichoderma reesei RUTC30

From: Involvement of the adaptor protein 3 complex in lignocellulase secretion in Neurospora crassa revealed by comparative genomic screening

No. Mutant (FGSC) Orthologs in N. crassa a RUT C30 targetsb Gene product names Protein conc. (mg/L) Increased vs WT (%)c
1 11701 NCU03998 jgi|Trire2|53811| Adaptor protein complex 3 Mu3A 821.38 ± 21.83 42.9
2 17965 NCU07880 jgi|Trire2|124172| Protein kinase 780.01 ± 32.87 35.64
3 19384 NCU01161 jgi|Trire2|68926| Actin polymerization protein Bzz1 773.06 ± 31.51 34.44
4 19393 NCU07492 jgi|Trire2|122689| Hypothetical protein 758.58 ± 21.01 32.21
5 15743 NCU01242 jgi|Trire2|69437| G2/mitotic-specific cyclin 759.76 ± 41.93 32.06
6 10372 NCU08807 jgi|Trire2|120117| cre-1 755.26 ± 51.79 31.22
7 11964 NCU09120 jgi|Trire2|76515| Lysine-specific histone demethylase Aof2 750.7 ± 20.09 30.6
8 15360 NCU06992 jgi|Trire2|31118| DNA repair protein Nse1 737.31 ± 37.42 28.59
9 16681 NCU04201 jgi|Trire2|106234| Signal transduction protein Syg1 729.66 ± 38.29 26.84
10 12039 NCU08441 jgi|Trire2|123786| Non-ribosomal peptide synthetase 729.27 ± 112.17 26.36
11 12276 NCU03145 jgi|Trire2|78301| Vacuolar membrane zinc transporter 723.79 ± 40.24 25.81
12 13177 NCU01838 jgi|Trire2|65106| Nitrilase 722.38 ± 13.8 25.71
13 11468 NCU04142 jgi|Trire2|123114| Heat shock protein 80 717.96 ± 59.51 24.68
14 12062 NCU07498 jgi|Trire2|79304| DNA excision repair protein Rad2 715.8 ± 57.68 24.32
15 16319 NCU02842 jgi|Trire2|121087| Hypothetical protein 712.55 ± 32.87 23.89
16 18830 NCU03811 jgi|Trire2|121453| Hypothetical protein 710.24 ± 91.52 23.16
17 19831 NCU03545 jgi|Trire2|82153| Hypothetical protein 706.55 ± 32.91 22.85
18 11956 NCU08809 jgi|Trire2|74765| Hypothetical protein 704.12 ± 19.44 22.5
19 16594 NCU03914 jgi|Trire2|64375| Glucan 1,3-beta-glucosidase 704.88 ± 64.43 22.38
20 20296 NCU08364 jgi|Trire2|78320| Choline sulfatase 702.59 ± 19.06 22.23
21 11040 NCU05411 jgi|Trire2|70071| Pathway-specific nitrogen regulator 701.46 ± 65.9 21.78
22 19539 NCU07334 jgi|Trire2|68425| Uracil permease 698.2 ± 25.84 21.72
23 18971 NCU00503 jgi|Trire2|71037| Nonselective cation channel protein 700.38 ± 66.13 21.59
24 13289 NCU00427 jgi|Trire2|80691| Hypothetical protein 698.5 ± 19.9 21.52
25 15939 NCU00754 jgi|Trire2|58561| Multidrug resistant protein 697.71 ± 79.14 21.05
26 13436 NCU05089 jgi|Trire2|64882| MFS monocarboxylate transporter 692.03 ± 30.99 20.33
27 16570 NCU04886 jgi|Trire2|28409| MFS multidrug transporter 693.08 ± 69.06 20.3
28 16331 NCU03068 jgi|Trire2|58790| Glycerol-3-phosphate phosphatase 1 690.98 ± 32.01 20.14
29 20407 NCU11050 jgi|Trire2|75105| DUF455 domain-containing protein 689.63 ± 40.55 19.86
30 11084 NCU09549 jgi|Trire2|26255| C6 zinc finger domain-containing protein 683.56 ± 79.47 18.58
31 18230 NCU09887 jgi|Trire2|67030| Drp1p 678.63 ± 42.93 17.93
32 19296 NCU07276 jgi|Trire2|67732| ABC bile acid transporter 677.02 ± 69.64 17.5
33 15452 NCU06309 jgi|Trire2|22841| Hypothetical protein 674.61 ± 18.59 17.36
34 15930 NCU00648 jgi|Trire2|59952| Choline transporter 675.67 ± 114.38 17.02
35 13757 NCU01420 jgi|Trire2|54157| Hypothetical protein 672.9 ± 36.3 16.97
36 15627 NCU04626 jgi|Trire2|28731| G-protein coupled receptor 667.67 ± 37.79 16.05
37 15627 NCU04626 jgi|Trire2|123806| G-protein coupled receptor 667.67 ± 37.79 16.05
38 16748 NCU00799 jgi|Trire2|40758| Homocysteine S-methyltransferase 669.17 ± 128.93 15.8
39 14141 NCU08499 jgi|Trire2|58161| GTPase-activating protein GYP5 663.7 ± 13.31 15.49
40 13568 NCU04521 jgi|Trire2|54511| Hypothetical protein 665.59 ± 127.62 15.19
41 20195 NCU06578 jgi|Trire2|104161| KapG 662.02 ± 104.79 14.69
42 19233 NCU01997 jgi|Trire2|74570| ABC transporter 662.83 ± 141.67 14.63
43 12060 NCU07381 jgi|Trire2|3027| DNA cross-link repair protein pso2/snm1 659.15 ± 93.95 14.25
44 18123 NCU07703 jgi|Trire2|55887| Hypothetical protein 655.25 ± 49.89 13.82
45 13910 NCU05195 jgi|Trire2|75072| Hypothetical protein 653.76 ± 52.14 13.55
46 11279 NCU03125 jgi|Trire2|79405| NIMA-interacting protein TinC 651.64 ± 26.12 13.32
47 12569 NCU00321 jgi|Trire2|67024| Hypothetical protein 652.72 ± 75.61 13.23
48 19403 NCU07564 jgi|Trire2|78465| Siderophore iron transporter mirC 648.04 ± 5.97 12.87
49 15566 NCU01961 jgi|Trire2|59147| DNA lyase Apn2 647.69 ± 69.51 12.39
50 19712 NCU00497 jgi|Trire2|58391| Hypothetical protein 643.14 ± 129.41 11.27
51 11150 NCU00278 jgi|Trire2|73912| Hypothetical protein 643.22 ± 144.06 11.2
52 11459 NCU00340 jgi|Trire2|36543| Transcription factor steA 637.2 ± 73.32 10.54
53 13984 NCU05837 jgi|Trire2|65104| Vacuolar protein sorting-associated protein 13a 635.14 ± 16.93 10.5
54 19183 NCU05477 jgi|Trire2|102776| Hypothetical protein 632.43 ± 18.1 10.02
55 11061 NCU08443 jgi|Trire2|77513| Transcription factor ace3 635.36 ± 137.21 9.87
56 13086 NCU00541 jgi|Trire2|80592| Hypothetical protein 627.2 ± 73.28 8.8
57 16803 NCU04809 jgi|Trire2|82037| MFS phospholipid transporter 630.47 ± 248.43 8.4
58 15478 NCU08642 jgi|Trire2|78268| Cyclic nucleotide-binding domain-containing protein 624.52 ± 83.42 8.28
59 15647 NCU00025 jgi|Trire2|82499| Integral membrane protein 623.56 ± 73.21 8.17
60 16280 NCU04108 jgi|Trire2|105874| Isoamyl alcohol oxidase 616.8 ± 25.92 7.26
61 15869 NCU00335 jgi|Trire2|5140| Pre-mRNA-splicing factor cwc15 612.18 ± 15.31 6.51
62 13160 NCU01633 jgi|Trire2|62380| Hexose transporter HXT13 611.29 ± 19.85 6.33
63 19733 NCU06832 jgi|Trire2|112231| Kinesin 609.8 ± 45.91 5.93
64 12341 NCU09864 jgi|Trire2|56726| 2-Oxoisovalerate dehydrogenase alpha subunit 608.18 ± 4.15 5.88
65 11030 NCU07788 jgi|Trire2|52368| Fungal specific transcription factor 606.86 ± 36.13 5.47
66 12282 NCU06647 jgi|Trire2|5403| Enoyl-CoA hydratase/isomerase 605.74 ± 7.76 5.43
67 12018 NCU02751 jgi|Trire2|120806| Serine/threonine-protein kinase 605.22 ± 59.32 5.05
68 11677 NCU01868 jgi|Trire2|59388| MFS maltose permease MalP 595.74 ± 3.43 3.71
69 18917 NCU05459 jgi|Trire2|65773| Mitochondrial AAA ATPase 595.8 ± 64.3 3.39
70 19405 NCU07574 jgi|Trire2|22294| Hypothetical protein 591.48 ± 5.41 2.96
71 17946 NCU04755 jgi|Trire2|45456| Protein kinase domain-containing protein ppk32 581.14 ± 12.76 1.12
72 17081 NCU01044 jgi|Trire2|63464| Hypothetical protein 580.08 ± 10.41 0.95
73 19059 NCU08307 jgi|Trire2|56077| Hypothetical protein 579.21 ± 100.55 0.3
74 17389 NCU02337 jgi|Trire2|80332| Mitochondrial carrier protein 576.12 ± 32.03 0.14
75 2489 ##### ##### Wild type 575.45 ± 36.81 0
76 16836 NCU04847 jgi|Trire2|52520| cyclin 573.97 ± 15.22 −0.13
77 20073 NCU06341 jgi|Trire2|44956| MFS transporter 573.58 ± 9.57 −0.17
78 19245 NCU02220 jgi|Trire2|64866| hypothetical protein 563.98 ± 19.59 −1.9
79 12078 NCU00523 jgi|Trire2|50268| NAD-dependent deacetylase sirtuin-2 551.74 ± 23.25 −4.05
80 12072 NCU04203 jgi|Trire2|121351| Glucosidase II alpha subunit 508.03 ± 35.04 −11.73
81 13475 NCU07119 jgi|Trire2|60458| Nonribosomal peptide synthase 2 503.65 ± 12.98 −12.37
82 19165 NCU05213 jgi|Trire2|75074| Hypothetical protein 449.4 ± 86.39 −22.22
83 18185 NCU08452 jgi|Trire2|110570| Hypothetical protein 439.95 ± 23.92 −23.52
84 16956 NCU02152 jgi|Trire2|3400| RRM domain-containing protein 430.62 ± 18.69 25.11
85 11357 NCU03184 jgi|Trire2|4921| C2H2 conidiation transcription factor FlbC 419.08 ± 52.77 27.31
86 20306 NCU08380 jgi|Trire2|122050| Plasma membrane phosphatase 416.5 ± 18.07 27.57
87 11360 NCU03244 jgi|Trire2|62053| WD repeat protein 346.47 ± 27.22 39.81
  1. a N. crassa orthologs: locus selected according to the N. crassa database (v7) (https://www.broadinstitute.org/annotation/genome/neurospora/MultiHome.html).
  2. bRUTC30 targets: locus selected according to the T. reesei genome sequence web site (http://genome.jgi-psf.org/Trire2/Trire2.home.html).
  3. cThe increased percentage of secreted protein relative to the WT. Strains more than 25% increased or decreased compared with the WT are shown in italics.