Table 5 Strains and plasmids

Strains

 E. coli

  DH5α

E. coli cloning strains

N/A

New England Biolabs

 T. saccharolyticum

  LL1025

Wild-type strain

SRA234880

[32]

  LL1040 (aka ALK2)

High ethanol-producing strain, Kan^r, Erm^r

N/A

[2]

  LL1049 (aka M1442)

High ethanol-producing strain

SRA233073

[4]

  LL1139

LL1025 ΔpforA ::Kan^r, colony 1

SRA234882

This study

  LL1140

LL1025 ΔpforA ::Kan^r, colony 2

SRA233066

This study

  LL1141

Adapted LL1139

SRA234883

This study

  LL1142

Adapted LL1140

SRA234884

This study

  LL1155

LL1025 ΔpforD ::‏ Kan^r,

N/A

This study

  LL1156

LL1025 ΔpforB :: ‏Kan^r,

N/A

This study

  LL1157

LL1025 ΔpforF :: ‏Kan^r,

N/A

This study

  LL1159

LL1049 ΔpforA :: ‏Kan^r,

N/A

This study

  LL1164

LL1025 Δpfl :: Kan^r, colony 1

SRA233080

This study

  LL1170

LL1025 Δpfl :: Kan^r, colony 2

SRA233074

This study

  LL1178

LL1141 Δpfl :: Erm^r

SRA234885

This study

Plasmids

 pMU433

Cloning vector pta/ack, Kan^r

[33]

 pZJ13

pforA knockout, Kan^r, pta/ack

KP057684

This study

 pZJ15

pforB knockout, Kan^r, pta/ack

KP057685

This study

 pZJ16

pforD knockout, Kan^r, pta/ack

KP057686

This study

 pZJ17

pforF knockout, Kan^r, pta/ack

KP057687

This study

 pZJ20

pfl knockout, Kan^r, pta/ack

KP057688

This study

 pZJ23

Cloning vector Erm^r, Amp^r

KP057689

This study

 pZJ25

pfl knockout, Erm^r, Amp^r

KP057690

This study