Skip to main content

Table 2 Detected enzymes relation to polysaccharide degradation by HPLC/MS

From: Characterization of hemicellulase and cellulase from the extremely thermophilic bacterium Caldicellulosiruptor owensensis and their potential application for bioconversion of lignocellulosic biomass without pretreatment

Entry name Detected sequence Gene name Enzyme MW kDa Signal peptide (aa)a Transmembrane domain (aa)b
Extra-enzymes cultivated on corncob xylan
 E4Q6K1_CALOW E.PVVIEF.L Calow_2098 β-galactosidase 118.37 No No
 E4Q2A1_CALOW G.VGGNNHHQ.L Calow_0121 β-xylanase 152.48 No 13–35
 E4Q5G9_CALOW Q.AYEGSY.S Calow_1924 β-xylanase 187.02 1–33 5–27
 E4Q4M1_CALOW E.DAILVGCM.L Calow_0926 α-N-arabinofuranosidase 57.89 No No
 E4Q281_CALOW P.EIAKLY.V Calow_0101 α-amylase catalytic region 66.17 No No
 E4Q359_CALOW G.YDPHDYYDLGQ.Y Calow_0294 α-amylase catalytic region 53.67 1–11 13–35
 E4Q4A4_CALOW L.VAPISMFVAYKSD.E Calow_0483 Pullulanase, type I 127.94 No 13–32
 E4Q6L6_CALOW G.VRISNC.Y Calow_2114 Glycoside hydrolase family 28 50.05 No No
 E4Q2L6_CALOW T.IVGGY.K Calow_0163 Glycoside hydrolase 15-related protein 70.76 No No
 E4Q1R5_CALOW T.KFALPIIL.S Calow_2141 Polysaccharide deacetylase 29.98 No No
 E4Q4M2_CALOW I.QVISALF.E Calow_1765 α-l-fucosidase 86.88 No No
 E4Q6L2_CALOW T.PGDSSV.F Calow_2109 Pectate disaccharide-lyase 188.76 No 28–50
 E4Q5E4_CALOW E.NPDPVL.V Calow_1899 Esterase/lipase-like protein 30.17 No No
Intra-enzymes cultivated on corncob xylan
 E4Q6Z2_CALOW T.YEEVMALVGHHLSLN.I Calow_2166 Glycoside hydrolase family 18 87.44 1–24 5–27
 E4Q4N4_CALOW V.VLVEK.G Calow_0544 Glycosidase-related protein 35.84 No No
 E4Q2L6_CALOW T.IVGGY.K Calow_0163 Glycoside hydrolase 15-related protein 70.76 No No
 E4Q4J8_CALOW W.SHDIAGFE.S Calow_1739 Glycoside hydrolase family 31 88.91 No No
 E4Q6K9_CALOW I.WAPAIRYHNGRFYIY.F Calow_2106 Glycoside hydrolase family 43 59.87 No No
 E4Q4G6_CALOW G.TVRLYDIDFEAAKTNEV
IGNKLSS.K
Calow_1700 Glycoside hydrolase family 4 52.72 No No
 E4Q6J2_CALOW P.VVSPER.Y Calow_0925 Glycoside hydrolase family 43 56.05 1–26 7–29
 E4Q1W6_CALOW K.NWIF.E Calow_0048 Glycoside hydrolase, family 20 63.68 No No
 E4Q347_CALOW N.YDEDEGF.I Calow_0282 Pullulanase, type I 96.51 No No
 E4Q4A4_CALOW Y.VSGTMN.D Calow_0483 Pullulanase, type I 127.94 No 13–32
 E4Q281_CALOW I.MYKWYLALKDKGWN.S Calow_0101 α-amylase catalytic region 66.17 No No
 E4Q4A2_CALOW V.AKVKVANLIQNSGF.E Calow_0481 Arabinogalactan endo-β-1,4-galactanase 90.88 No No
 E4Q4L3_CALOW P.QWHMK.W Calow_1754 α-l-fucosidase 56.75 No No
  1. aSignal peptides were predicted by SignalP 4.0
  2. bTransmembrane domains were predicted by TMHMM server v. 2.0