Fig. 1

Monomeric glucose (left) and xylose (right) saccharification yields as a function of milling time. Five grams of UT-2 spring-harvested 1-mm knife-milled switchgrass was milled at room temperature using 200 g of 0.25″ stainless steel beads in a 125-mL plastic bottle at an end-to-end rotation speed of 83 rotations/min. Saccharification was performed for the indicated times (in hours) in 50 mM citrate buffer, pH 4.9 with Accellerase 1500 (25 mg protein/g glucan) + a beta xylosidase cocktail (16.6 mg protein/g xylan) at 47 °C using a 14 % solids loading. At 120 h, of saccharification, both the monomeric and the monomeric plus oligomeric (total) sugar yields are indicated