Fig. 6

Uptake of ¹⁴C-labelled glucose by the yeast hexose transporter null mutant EBY.VW4000 expressing mstG or mstH as a function of glucose concentration. a, b Diamonds represent measured uptake rates of ¹⁴C labelled glucose (n = 3) as a result of expression of mstG (a) or mstH (b). Black lines represent least-squares-fitted Michaelis–Menten model (mstG; K _m = 0.5 mM; V _max = 5.8 nmol min⁻¹ mg DW⁻¹; mstH: K _m = 0.06 mM, and a V _max = 1.3 nmol min⁻¹ mg DW⁻¹). Insert results for mstH using micro-molar substrate concentrations. c Uptake rate of 1 mM ¹⁴C-labelled glucose by EBY.VW4000 expressing mstG (filled boxes) or mstH (open boxes) in the presence of a tenfold excess of competing sugars