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Table 1 Glucose consumption, product and yield analyses of E. coli strains harboring different vectors in 24 h flask cultures

From: Metabolic engineering of Escherichia coli for production of (2S,3S)-butane-2,3-diol from glucose

Strain

Glucose consumed (g/L)

2,3-BD (g/L)

2,3-BD yield (g/g)

E. coli BL21 (pETDuet-1)

12.00 ± 0.00

ND

0.00

E. coli BL21 (pET28a–lysR–PabcbudBbudC)

14.00 ± 0.00

ND

0.00

E. coli BL21 (pETDuet–PT7budB–PT7budC)

13.67 ± 0.58

1.14 ± 0.01

0.08

  1. Data are the mean ± standard deviations (SDs) from three parallel experiments