Table 4 Researches on the production of (2S,3S)-2,3-BD using varying substrates
From: Metabolic engineering of Escherichia coli for production of (2S,3S)-butane-2,3-diol from glucose
Biocatalyst | Substrate | (2S,3S)-2,3-BD (g/L) | Yield (g/g) | Co-substrate | Cost ($/kg) | References |
|---|---|---|---|---|---|---|
E. coli coexpressing formate dehydrogenase from Candida boidinii NCYC 1513 and meso-BDH from E. cloacae subsp. dissolvens SDM | Diacetyl | 31.7 | 0.90 | Formate | 24.4 | [23] |
E. coli expressing meso-BDH from E. cloacae subsp. dissolvens SDM | Diacetyl | 26.8 | 0.67 | Glucose | 32.8 | [24] |
E. coli JM109 coexpressing meso-BDH from K. pneumoniae and (2S,3S)-2,3-BDH from Brevibacterium saccharolyticum C-1012 | Diacetyl | 2.2 | 0.93 | Glucose | 23.7 | [25] |
E. coli JM109 expressing (2S,3S)-2,3-BDH from Br. saccharolyticum | Racemic acetoin | 3.7 | 0.37 | Glucose | 85.7 | [26] |
E. coli expressing acetoin reductase from Rhodococcus erythropolis WZ010 | Diacetyl | 5.3 | 1.03 | NADH | 21.4 | [27] |
E. coli BL21 coexpressing (2R,3R)-2,3-BDH from Bacillus subtilis 168 and NADH oxidase from Lactobacillus brevis CICC 6004 | 2,3-BD | 2.4 | 0.12 | 13.3 | [28] | |
E. coli BL21 (DE3) coexpressing ALS and meso-BDH from E. cloacae subsp. dissolvens SDM | Glucose | 2.2 | 0.08 | 9.4 | This work |