Fig. 2
Comparison of gene expression levels of Z. mobilis ZM4 between the DNA microarray and qRT-PCR. The gene expression ratios of both microarray data and qRT-PCR data for 20 genes were log transformed in base 2 (log2, treatment/control), and the microarray log2 ratio values were plotted against the qRT-PCR log2 values. The 20 selected genes included ZMO0152 (pyruvate kinase), ZMO0177 (16S rRNA), ZMO0179 (fructose-bisphosphate aldolase), ZMO0367 (glucose-6-phosphate 1-dehydrogenase), ZMO0368 (6-phosphogluconate dehydrogenase), ZMO0369 (glucokinase), ZMO0387 (hpcH/hpaI aldolase), ZMO0543 (aconitate hydratase), ZMO0544 (isocitrate dehydrogenase), ZMO0567 (succinyl-CoA synthetase), ZMO0569 (succinate dehydrogenase), ZMO0997 (KDPG aldolase), ZMO1237 (d-isomer specific 2-hydroxyacid dehydrogenase), ZMO1307 (fumarase), ZMO1360 (pyruvate decarboxylase), ZMO1478 (6-phosphogluconolactonase), ZMO1496 (phosphoenolpyruvate carboxylase), ZMO1596 (Fe-containing alcohol dehydrogenase), ZMO1608 (phosphopyruvate hydratase), and ZMO1963 (citrate synthase). The primers for qRT-PCR of the genes are listed in Additional file 1