Table 3 Bacterial strains and plasmids used in this study

Strains

 K. oxytoca M1

Parent strain

[26]

 K. oxytoca M2

K. oxytoca M1 ΔpduC

This study

 K. oxytoca M3

K. oxytoca M1 ΔpduCΔldhA

This study

 K. oxytoca KCTC1686

Type strain

[33]

 E. coli DH5α

supE44, ΔlacU169, (φ80lacZΔM15), hsdR17, recA1, endA1, gyrA96, thi-1, relA1

Invitrogen

Plasmids

 pRedET

Derivative of pSC101, Tet^R, temperature sensitive, carrying lambda red recombinase

Gene bridge

 707-FLPe

Derivative of pSC101, Tet^R, temperature sensitive, containing an FLPe recombinase

Gene bridge

 pTOP-FCF

Derivative of pUC, containing an FRT-flanked Cm^R cassette-involved vector

[34]