A novel biocatalyst for efficient production of 2-oxo-carboxylates using glycerol as the cost-effective carbon source

Table 3 Comparison of 2-OBA productions by whole cells of P. putida KT2440, P. putida KT2440/pBSPPc_Gm-vgb, P. putida KT2440 (ΔlldR), and P. putida KT2440 (ΔlldR)/pBSPPc_Gm-vgb

Strain	2-OBA (mM)	Yield (%)^a	Productivity (mmol/g DCW h)
KT2440	0	0	0
KT2440/pBSPPc_Gm-vgb	0	0	0
KT2440 (ΔlldR)	75.52 ± 1.59	74.7	1.20 ± 0.03
KT2440 (ΔlldR)/pBSPPc_Gm-vgb	99.31 ± 1.45	99.8	1.58 ± 0.02

The initial dl-2-HBA concentration was 100 mM. The biocatalysis reactions were conducted at 30 °C in phosphate buffer (pH 7.4) for 6 h with 10.5 g DCW/L of biocatalysts prepared from glycerol. Results are mean ± SD of three parallel replicates
^aThe yields of 2-OBA were calculated based on the actual initial concentrations of dl-2-HBA measured by HPLC

ISSN: 2731-3654