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Table 3 Comparison of 2-OBA productions by whole cells of P. putida KT2440, P. putida KT2440/pBSPPcGm-vgb, P. putida KT2440 (ΔlldR), and P. putida KT2440 (ΔlldR)/pBSPPcGm-vgb

From: A novel biocatalyst for efficient production of 2-oxo-carboxylates using glycerol as the cost-effective carbon source

Strain 2-OBA (mM) Yield (%)a Productivity (mmol/g DCW h)
KT2440 0 0 0
KT2440/pBSPPcGm-vgb 0 0 0
KT2440 (ΔlldR) 75.52 ± 1.59 74.7 1.20 ± 0.03
KT2440 (ΔlldR)/pBSPPcGm-vgb 99.31 ± 1.45 99.8 1.58 ± 0.02
  1. The initial dl-2-HBA concentration was 100 mM. The biocatalysis reactions were conducted at 30 °C in phosphate buffer (pH 7.4) for 6 h with 10.5 g DCW/L of biocatalysts prepared from glycerol. Results are mean ± SD of three parallel replicates
  2. aThe yields of 2-OBA were calculated based on the actual initial concentrations of dl-2-HBA measured by HPLC