Fig. 5
Recalcitrance study of neutral and acidic oligosaccharides. a Recalcitrance of crude oligosaccharides after charcoal fractionation based on monomeric sugar composition; b recalcitrance of oligosaccharides fractions separated by SEC determined. Enzymatic hydrolysis was performed in 0.5 mL reaction volumes with commercial enzymes (Ctec2:Htec2:MP) in 1:1:1 ratio for 24 h hydrolysis at 50 °C and pH 4.8. Maximum sugar concentration per well was 3 g/L while the minimum was 0.2 g/L (determined by acid hydrolysis). Enzyme loading is 60 μg/well