Fig. 1
From: Single-cell screening of photosynthetic growth and lactate production by cyanobacteria
Experimental workflow. a Lactate-producing cyanobacteria are encapsulated as single cells in 10 pL droplets. Following incubation to produce lactate, the droplets are injected with an enzyme assay that catalyzes the activation of a fluorescent dye in the presence of lactate. The fluorescent droplets are analyzed and sorted based on the strength of the signal. b Titration of pure lactate analyzed according to the workflow in a (yellow triangles) and using microtiter plate (blue squares). c Time-lapse experiment of lactate-producing strains (n = 10,000 droplets analyzed per time point, y-axis is broken). Vertical continuous and dashed lines mark the 99th and 90th percentiles, respectively. d Analysis of data in c, showing the 99th percentile (continuous lines, filled markers) and 90th percentile (dashed lines, open markers) for SAW035 (gray squares) and SAA005 (red circles)