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Table 1 Optical densities at 600 nm, H2 production, and concentrations of main metabolites at beginning and end of cultivations of C. butyricum and A. baylyi ADP1 gcd knockout strain

From: Metabolic engineering of Acinetobacter baylyi ADP1 for removal of Clostridium butyricum growth inhibitors produced from lignocellulosic hydrolysates

  C. butyricum (anaerobic) C. butyricum A. baylyi C. butyricum and A. baylyi
0 h 24 h 0 h 24 h 0 h 24 h 0 h 24 h
OD600 0.5 ± 0.0 >3.0 0.5 ± 0.0 0.4 ± 0.0 0.2 ± 0.0 1.1 ± 0.0 0.7 ± 0.1 >3.0
H2 (mM) 93.6 ± 3.6 0.0 ± 0.0 0.0 ± 0.0 92.0 ± 1.5
cGlucose (mM) 45.8 ± 1.1 15.5 ± 0.6 45.4 ± 0.5 46.1 ± 1.0 45.5 ± 0.7 45.0 ± 0.4 45.0 ± 0.4 5.5 ± 0.1
cCellobiose (mM) 34.5 ± 0.9 17.6 ± 0.1 34.6 ± 0.4 34.7 ± 0.7 34.7 ± 0.5 34.6 ± 0.3 34.2 ± 0.2 19.4 ± 0.3
cXylose (mM) 40.5 ± 1.0 38.3 ± 0.6 40.5 ± 0.4 40.9 ± 0.6 40.2 ± 0.6 39.2 ± 0.4 40.0 ± 0.3 36.9 ± 0.6
cAcetate (mM) 1.9 ± 0.1 27.5 ± 0.1 1.8 ± 0.1 2.2 ± 0.3 1.2 ± 0.0 0.2 ± 0.2 1.8 ± 0.1 28.6 ± 0.6
cButyrate (mM) 0.7 ± 0.0 31.9 ± 0.3 0.7 ± 0.0 0.9 ± 0.3 0.0 ± 0.0 0.0 ± 0.0 0.7 ± 0.1 34.9 ± 0.7
cCitrate (mM) 34.4 ± 0.8 29.9 ± 0.4 34.9 ± 0.4 35.3 ± 0.7 34.1 ± 0.5 31.7 ± 0.3 33.8 ± 0.3 29.5 ± 0.6
  1. Results shown are averages with standard deviations from triplicate cultivations