Metabolic engineering of Acinetobacter baylyi ADP1 for removal of Clostridium butyricum growth inhibitors produced from lignocellulosic hydrolysates

Table 1 Optical densities at 600 nm, H₂ production, and concentrations of main metabolites at beginning and end of cultivations of C. butyricum and A. baylyi ADP1 gcd knockout strain

	C. butyricum (anaerobic)		C. butyricum		A. baylyi		C. butyricum and A. baylyi
	0 h	24 h	0 h	24 h	0 h	24 h	0 h	24 h
OD₆₀₀	0.5 ± 0.0	>3.0	0.5 ± 0.0	0.4 ± 0.0	0.2 ± 0.0	1.1 ± 0.0	0.7 ± 0.1	>3.0
H₂ (mM)	‒	93.6 ± 3.6	‒	0.0 ± 0.0	‒	0.0 ± 0.0	‒	92.0 ± 1.5
c_Glucose (mM)	45.8 ± 1.1	15.5 ± 0.6	45.4 ± 0.5	46.1 ± 1.0	45.5 ± 0.7	45.0 ± 0.4	45.0 ± 0.4	5.5 ± 0.1
c_Cellobiose (mM)	34.5 ± 0.9	17.6 ± 0.1	34.6 ± 0.4	34.7 ± 0.7	34.7 ± 0.5	34.6 ± 0.3	34.2 ± 0.2	19.4 ± 0.3
c_Xylose (mM)	40.5 ± 1.0	38.3 ± 0.6	40.5 ± 0.4	40.9 ± 0.6	40.2 ± 0.6	39.2 ± 0.4	40.0 ± 0.3	36.9 ± 0.6
c_Acetate (mM)	1.9 ± 0.1	27.5 ± 0.1	1.8 ± 0.1	2.2 ± 0.3	1.2 ± 0.0	0.2 ± 0.2	1.8 ± 0.1	28.6 ± 0.6
c_Butyrate (mM)	0.7 ± 0.0	31.9 ± 0.3	0.7 ± 0.0	0.9 ± 0.3	0.0 ± 0.0	0.0 ± 0.0	0.7 ± 0.1	34.9 ± 0.7
c_Citrate (mM)	34.4 ± 0.8	29.9 ± 0.4	34.9 ± 0.4	35.3 ± 0.7	34.1 ± 0.5	31.7 ± 0.3	33.8 ± 0.3	29.5 ± 0.6

Results shown are averages with standard deviations from triplicate cultivations

ISSN: 2731-3654