Table 4 The total percent loss in pNP activity due to the proteins being bound to lignin at different pH and ionic strengths of the buffering solution
From: New perspective on glycoside hydrolase binding to lignin from pretreated corn stover
pNP substrates | Activity loss in pNP activity in the unbound fraction at different pH and salt concentrations | |||
|---|---|---|---|---|
pH 4.8 | pH 6.0 | |||
50 mM | 300 mM | 50 mM | 300 mM | |
pNP-cellobioside (β-d-glucosidases, endoglucanases) | 87 (%) | 81 (%) | 73 (%) | 65 (%) |
pNP-xylopyranoside (xylanases; β-d-xylobiosidases) | 76 (%) | 47 (%) | 72 (%) | 37 (%) |