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Table 4 The total percent loss in pNP activity due to the proteins being bound to lignin at different pH and ionic strengths of the buffering solution

From: New perspective on glycoside hydrolase binding to lignin from pretreated corn stover

pNP substrates Activity loss in pNP activity in the unbound fraction at different pH and salt concentrations
pH 4.8 pH 6.0
50 mM 300 mM 50 mM 300 mM
pNP-cellobioside (β-d-glucosidases, endoglucanases) 87 (%) 81 (%) 73 (%) 65 (%)
pNP-xylopyranoside (xylanases; β-d-xylobiosidases) 76 (%) 47 (%) 72 (%) 37 (%)