Fig. 1
From: Multifunctional cellulase catalysis targeted by fusion to different carbohydrate-binding modules
Schematics of the plasmid and fusion proteins used in this work. a Schematic of the plasmid and nucleotides in the functional region of pEUTTJW used to create fused gene sequences for cell-free protein translation. Locations of flanking primer pair used to transfer an assembled fusion protein into pVP67K for expression in E. coli are shown as circles 1 and 2 (blue lines). b Schematic of the domain structures of expressed protein consisting of either GFP (green) or CelE (purple), followed by the linker (blue) and the CBM domain (yellow)