Fig. 4

Hydrolytic activity of CelEcc_CBMs with purified polysaccharides. CelEcc_CBMs prepared using cell-free translation and tested, without purification from the translation reaction, for hydrolysis of phosphoric acid-swollen cellulose (PASC), lichenan, beechwood xylan, and mannan. A red star indicates a CelEcc_CBM hybrid that had a 200 % or more increase in activity relative to starting benchmark CelEcc_CBM3a (green filled circle). Diamonds indicate GFP_CBMs that bound to the indicated substrate according to binding experiments. Results with CBM3a, CBM6, CBM22-2, CBM30, and CBM44 are colored green, purple, yellow, magenta, and orange, respectively. Subsequent experiments focused on these five CBMs. Error bars indicate ±1 standard deviation from three independent experiments