Fig. 5
From: Multifunctional cellulase catalysis targeted by fusion to different carbohydrate-binding modules
Binding-affinity plots for GFP_CBM fusions. GFP_CBMs used in this experiment were expressed in E. coli and purified as described in “Methods”. The fraction bound (y-axis) versus substrate concentration (x-axis) are shown for three different insoluble substrates. The plots were used to determine dissociation constants with the binding model given in Eq 6. Shaded regions around the plotted affinity curve are the mean prediction bands at the 90 % confidence level. a PASC plot and data fitting (GFP_CBM3a, brown; GFP_CBM30, blue; GFP_CBM44, red). b Lichenan plot and data fitting (GFP_CBM6, green; GFP_CBM30, blue; GFP_CBM44, red). c Xylan plot and data fitting (GFP_CBM6, green; GFP_CBM44, red)