Fig. 5
Phenotypes of the amylase and granulose mutants and complemented clones. I A 20 µl aliquot of a normalised overnight culture was spotted on CBM agar containing 2 % (w/v) starch, 0.5 % Glucose and plate stained with iodine potassium iodide solution after 48 h. A CRG4897, carrying a functional copy of amyP under the control of the Pfdx promoter inserted into the genome using the ACE vector pMTL-ME6X::amyP. B C. acetobutylicum ATCC 824 COSMIC strain wild-type. C CRG4896, carrying a functional copy of amyP and its own promoter inserted into the genome using the ACE vector pMTL-ME6C::amyP. D CRG4894, ΔamyP mutant. II All strains were grown on CBM agar plates with 5 % glucose to promote granulose formation and exposed to iodine vapours after 72 h. A CRG4898, ΔglgA mutant; B CRG4901 carrying a functional copy of glgA and its own promoter inserted into the genome using the ACE vector pMTL-ME6C::glgA; C CRG4902 carrying a functional copy of glgA under the control of the Pfdx promoter inserted into the genome using the ACE vector pMTL-ME6X::glgA. D C. acetobutylicum ATCC 824 COSMIC strain wild-type