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Table 1 Strains and plasmids used in this study

From: Mutant generation by allelic exchange and genome resequencing of the biobutanol organism Clostridium acetobutylicum ATCC 824

Strain/plasmid Relevant features Reference/source
Strains
 E. coli Top10 F- mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 deoR recA1 araD139 Δ(ara-leu)7697 galU galK rpsL (StrR) endA1 nupG Invitrogen
 C. acetobutylicum
ATCC 824 (COSMIC)
CRG1268 strain used in the ERANET (http://www.sysmo.net) SysMO project COSMIC. Originally from G. Bennet, RICE University, USA H Bahl University of Rostock
 C. acetobutylicum
ATCC 824
CRG3286, resourced from the ATCC in November 2011 ATCC
 C. acetobutylicum
CRG1545
ΔpyrE mutant of CRG1268 made using the ACE vector pMTL-JH12 [24]
 C. acetobutylicum
CRG3899
ΔpyrE mutant of CRG3286 equivalent to CRG1268 This study
 C. acetobutylicum
CRG3520
Δspo0A of CRG1545 (ΔpyrE) made using pMTL-ME3::spo0A This study
 C. acetobutylicum
CRG4889
Δspo0A mutant of CRG1545 generated from CRG3520 by restoration of the pyrE allele to wild-type using pMTL-ME6 This study
 C. acetobutylicum
CRG4890
CRG3520 (Δspo0A) carrying a functional copy of spo0A and its own promoter inserted into the genome using the ACE vector pMTL-ME6X::spo0A concomitant with restoration of the pyrE allele This study
 C. acetobutylicum CRG4891 CRG3520 (Δspo0A) carrying a functional copy of spo0A under the control of the fdx promoter inserted into the genome using the ACE vector pMTL-ME6X::spo0A concomitant with restoration of pyrE This study
 C. acetobutylicum
CRG4893
In-frame deletion mutant of amyP (CaP0168) made using pMTL-ME3::amyP (pyrE vector with amyP KO cassette) This study
 C. acetobutylicum
CRG4894
restoration of the pyrE allele to wild-type using pMTL-ME6 This study
 C. acetobutylicum
CRG4896
CRG4893 (ΔamyP) carrying a functional copy of amyP and its own promoter inserted into the genome using the ACE vector pMTL-ME6C::amyP concomitant with restoration of the pyrE allele This study
 C. acetobutylicum
CRG4897
CRG4894 (ΔamyP) carrying a functional copy of amyP under the control of the fdx promoter inserted into the genome using the ACE vector pMTL-ME6X::amyP concomitant with restoration of pyrE allele This study
 C. acetobutylicum
CRG4883
In-frame deletion mutant of glgA (Cac2239) made using pMTL-ME3::glgA (pyrE vector with glgA KO cassette) This study
 C. acetobutylicum
CRG4898
ΔglgA mutant of CRG4883 generated from CRG1545 by restoration of the pyrE allele to wild-type using pMTL-ME6 This study
 C. acetobutylicum
CRG4901
CRG4883 (ΔglgA) carrying a functional copy of glgA and its own promoter inserted into the genome using the ACE vector pMTL-ME6C::glgA concomitant with restoration of the pyrE allele This study
 C. acetobutylicum
CRG4902
CRG4883 (ΔglgA) carrying a functional copy of glgA under the control of the fdx promoter inserted into the genome using the ACE vector pMTL-ME6X::glgA concomitant with restoration of pyrE allele This study
 C. acetobutylicum CRG2404 In-frame deletion mutant of spo0A gene made using pMTL-SC7515::spo0A This study
 C. acetobutylicum CRG2403 In-frame deletion mutant of the type II Restriction system Cac1502 made using pMTL-SC7515::cac824I This study
Plasmids   
 pAN-2 E.coli vector (ϕ3T I methyltransferase gene, tet, p15a) CRG685 [13]
 pMTL85141 E.coli- Clostridium Shuttle vector (pIM13 catP ColEI) CRG1000 [31]
 pMTL-ME2 Clostridium KO vector (pCB102 catP ColE1 pyrE) CRG1668 [25]
 pMTL-ME3 Clostridium KO vector (pIM13 catP ColE1 pyrE) CRG1671This study
 pMTLSC7315 Clostridium KO vector (pCB102 catP ColE1 traJ codA) CRG1424 [21]
 pMTLSC7515 Clostridium KO vector (pIM13 catP ColE1 traJ codA) CRG1303 This study
 pMTL-ME6 C. acetobutylicum ACE correction vector (pIM13 catP ColE1 `pyrE) CRG1861 This study
 pMTL-ME6C C. acetobutylicum ACE complementation vector (pIM13 catP ColE1 pyrE lacZ’ MCS) CRG2483 [25]
 pMTL-ME6X C. acetobutylicum ACE expression vector (pIM13 catP ColE1 `pyrE P fdx, lacZ’ MCS) CRG2482 [25]
 pMTL-ME3::spo0A pMTL-ME3 + spo0A KO cassette (pIM13 catP ColE1 pyrE) CRG1701 This study
 pMTL-ME3::amyP pMTL-ME3 + amyP KO cassette (pIM13 catP ColE1 pyrE) CRG3727 This study
 pMTL-ME3::glgA pMTL-ME3 + glgA KO cassette (pIM13 catP ColE1 pyrE) CRG4881 This study
 pMTL-SC7515::spo0A pMTLSC7515 + spo0A KO cassette (pIM13 catP ColE1 codA) CRG1672 This study
 pMTL-SC7515::cac824I pMTLSC7515 + cac824I KO cassette (pIM13 catP ColE1 codA) CRG2293 This study
 pMTL-ME6C::spo0A ACE complementation vector for spo0A CRG3739 This study
 pMTL-ME6X::spo0A ACE overexpression vector for spo0A CRG3738 This study
 pMTL-ME6C::amyP ACE complementation vector for amyP CRG3723 This study
 pMTL-ME6X::amyP ACE overexpression vector for amyP CRG3722 This study
 pMTL-ME6C::glgA ACE complementation vector for glgA CRG4900 This study
 pMTL-ME6X::glgA ACE overexpression vector for glgA CRG4880 This study