Fig. 2
From: Overcoming substrate limitations for improved production of ethylene in E. coli
a Peak production of ethylene from high-, medium-, and low-copy plasmids in LB media, M9 (0.2 % glucose) and MOPS (0.2 % glucose) minimal media. b Western blots of EFE-Flag proteins expressed in E. coli (MG1655) from high-, medium-, and low-copy plasmids in different media. In each instance a significant amount of expressed protein remained insoluble. W whole cell; S soluble; P lysed cell pellet