3D-TEM reconstruction and volumetric analysis of Parachlorella kessleri.
a–c 3D-TEM image of a control cell from log-phase culture (stress-free conditions). d–f 3D-TEM image of a starch-rich phase cell (stressed conditions). g–i 3D-TEM image of a lipid-rich phase cell (stressed conditions). j–l Relative volumes of subcellular components and organelles in control cells, starch-rich, and lipid-rich cells, respectively. In each stage, 3D images are shown as a whole-cell, a whole-cell without chloroplast, and lipid body only. Volumes in the pie charts are the means of two representative cells of each stage. All subcellular and graph components are denoted by pseudocolors as indicated in the color legends. See also Additional files 2, 3, 4, and 5 as an image-rotation movie