Predicting compositions of microbial communities from stoichiometric models with applications for the biogas process

Table 3 Estimated flux rates and steady-state biomass compositions from Meyer et al. [36, 48] for growth of D. alaskensis with M. maripaludis or M. hungatei on pyruvate and lactate medium [36]

Substrate	Growth rate (h⁻¹)	Specific flux rates (mmol/g_DW/h)			F _DV (−)
Substrate	Growth rate (h⁻¹)	r_{pyruvate/lactate}	r_acetate	r_methane	F _DV (−)
Pyruvate	0.027	6.6–7.4	5.9–6.9	1.6–1.8	0.79
	0.027	(5.36)	(5.02)	(1.09)	(0.83)
	0.047	11.4–12.9	10.5–11.9	2.7–3.0	0.77
	0.047	(6.99)	(6.36)	(1.36)	(0.86)
Lactate	0.027	9.13	8.22	4.34–5.79	0.39
	0.027	(8.17)	(8.00)	(3.67)	(0.42)
	0.047	16.47	14.82	7.49–8.99	0.47
	0.047	(11.60)	(11.26)	(5.12)	(0.48)

Flux rates for hydrogen, carbon monoxide, and succinate were not considered for the simulations because they had only very small values (<0.2 mmol/g/h for succinate and <0.005 mmol/g/h for H₂ and CO). The model predictions are given in italics in brackets. We used ATPmaint = 4.3 mmol_ATP/gDW/h for D. vulgaris and ATPmaint = 0.9 mmol_ATP/gDW/h for M. maripaludis. The predicted community composition (F _DV) was determined by fixing the growth rate to the value of the experiment and then taking the composition with OptDeg = 1

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