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Fig. 2 | Biotechnology for Biofuels

Fig. 2

From: Dam and Dcm methylations prevent gene transfer into Clostridium pasteurianum NRRL B-598: development of methods for electrotransformation, conjugation, and sonoporation

Fig. 2

Testing the presence of potential restriction barriers. Cultivation of pMTL83253 (a) and pMTL82254 (b) with crude protoplast extract (PE) and whole cell lysate (WL) prepared from C. pasteurianum NRRL B-598 at 37 °C. Positive control (c): cultivation of pMTL83253 with PE prepared from C. pasteurianum DSM 525 by the same method

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