Fig. 2From: Dam and Dcm methylations prevent gene transfer into Clostridium pasteurianum NRRL B-598: development of methods for electrotransformation, conjugation, and sonoporationTesting the presence of potential restriction barriers. Cultivation of pMTL83253 (a) and pMTL82254 (b) with crude protoplast extract (PE) and whole cell lysate (WL) prepared from C. pasteurianum NRRL B-598 at 37 °C. Positive control (c): cultivation of pMTL83253 with PE prepared from C. pasteurianum DSM 525 by the same methodBack to article page