Metagenomic analysis of microbial consortia enriched from compost: new insights into the role of Actinobacteria in lignocellulose decomposition

Table 1 Glycoside hydrolase profiles targeting plant structural polysaccharides in four metagenomes

	Predominant activity	Termite [11]	Bovine [10]	Macropod [30]	RSA
Cellulases
GH5	Cellulases	7.97	1.23	4.31	2.36
GH6	Endoglucanases	0.00	0.00	0.18	0.77
GH7	Endoglucanases	0.00	0.00	0.00	0.00
GH9	Endoglucanases	1.28	0.92	0.36	1.12
GH44	Endoglucanases	0.85	0.00	1.08	0.06
GH45	Endoglucanases	0.57	0.00	0.00	0.00
GH48	Cellobiohydrolases	0.00	0.00	0.00	0.30
Subtotal (%)		10.67	2.15	5.92	4.60
Endohemicellulases
GH8	Endoxylanases	0.71	0.61	0.36	0.00
GH10	Endo-1,4-β-xylanases	6.54	1.08	2.51	2.18
GH11	Xylanases	1.99	0.15	0.00	0.47
GH12	Xyloglucanases	0.00	0.00	0.00	0.41
GH26	β-mannanase & xylanases	2.13	0.77	2.33	0.83
GH28	Galacturonases	0.85	0.77	0.54	0.65
GH53	Endo-1,4-β-galactanases	1.71	2.61	1.62	0.35
Subtotal (%)		13.94	5.99	7.36	4.90
Debranching enzymes
GH51	α-L-arabinofuranosidases	2.56	9.83	2.33	1.42
GH54	α-L-arabinofuranosidases	0.00	0.15	0.00	0.00
GH62	α-L-arabinofuranosidases	0.00	0.00	0.00	0.35
GH67	α-glucuronidases	1.42	0.00	0.90	0.35
GH78	α-L-rhamnosidases	0.00	5.22	4.49	2.30
Subtotal (%)		3.98	15.21	7.72	4.43
Oligosaccharide-degrading enzymes
GH1	β-glucosidases	3.13	1.54	11.13	3.72
GH2	β-galactosidases	3.27	28.57	5.03	1.59
GH3	β-glucosidases	9.82	27.04	13.46	4.90
GH29	α-L-fucosidases	0.00	11.37	0.54	0.89
GH35	β-galactosidases	0.43	1.84	0.72	0.41
GH38	α-mannosidases	1.56	2.61	0.54	0.77
GH39	β-xylosidases	0.43	0.31	0.18	1.89
GH42	β-galactosidases	3.41	1.69	1.62	0.83
GH43	Arabino/xylosidases	2.28	9.37	3.41	3.01
GH52	β-xylosidases	0.43	0.00	0.00	0.18
Subtotal (%)		24.75	84.33	36.62	18.18
Total GHs		703	651	557	1694
% ORFS		0.78	0.78	0.71	0.97

The listed value is the population abundance weighted relative abundance (%) of GH families among the total GHs

ISSN: 2731-3654