Fig. 2
Diagram of 10 L reactor system highlighting the satellite reactor and the main reactor. Control and all monitoring except temperature are carried out in the satellite reactor. Peristaltic pump 1 was set to a specified flow rate and pump 2 was matched by a scale feedback loop to maintain the mass in the satellite reactor for a total main tank turnover rate of 3/h. Fermentations were carried out at 25 % TS, 800 rpm agitation in the satellite tank, pH 5, and 35 °C for 144 h with feeding lasting 60 h