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Table 1 Includes data on ethanol yield, QS, viscosity, settling, the uronic acid assay, and enzymatic hydrolysis

From: Physical and chemical differences between one-stage and two-stage hydrothermal pretreated hardwood substrates for use in cellulosic ethanol production

Substrate MS1112 MS1105/MS1107
Parameter
 Ethanol yield   
  2 L—75 rpm (w/w) 0.359 0.286
  2 L—800 rpm (w/w) 0.384 0.340
  10 L—800 rpm (w/w) 0.313 0.287
 Quantitative saccharification (QS)
  Solids% cellulose (w/w) 63.88 ± 0.05 58.02 ± 0.03
  Solids% hemicellulose (w/w) 0 7.90 ± 0.08
  Solids% lignin (w/w) 28.0 33.0
 Viscosity (cP)
  Raw (10 % solids) 420 ± 10 55 ± 5
  Raw + ethanol (10 % solids) 545 ± 6 53 ± 5
  Chlorite extraction (10 % solids) 27 ± 1 13.4 ± 0.01
  Hydroxymethylfurfural (g/L) 74.55 84.00
 Sedimentation volume%
  10 % solids, 10 min 91 ± 1 36 ± 1
  10 % solids, 24 h 80 ± 2 35 ± 1
  20 % solids, 10 min 99.9 ± 0.1 76 ± 1
  20 % solids, 24 h 99.9 ± 0.1 65 ± 1
 Uronic acid assay (relative absorbance) 24.75 ± 0.09 9 ± 3
 Enzymatic hydrolysis (%)
  Pretreated hardwood 33.2 32.1
  Chlorite-treated PHW 36.2 18.9
  1. These results highlight the physical and chemical differences between the two substrates. MS1112 exhibits a higher ethanol yield and titer than MS1107 at all time points and conditions tested. MS1112 also exhibits increased sugar concentration, decreased lignin concentration, increased viscosity, increased settled volume, increased relative absorbance which indicates greater polarity, equivalent enzymatic sugar release with raw samples, and greater enzymatic sugar release with chlorite-pretreated samples