Anoxic metabolism and biochemical production in Pseudomonas putida F1 driven by a bioelectrochemical system

Table 2 Key process parameters of anaerobic glucose conversion of P. putida F1 in the anode compartment of a BES using [Co(bpy)₃]^3+/2+ or [Fe(CN)₆]^3−/4− as electron acceptors with the anode potential poised at +0.697 V vs SHE

	[Co(bpy)₃]^3+/2+	[Fe(CN)₆]^3−/4−
Carbon balance (%)	99.6	97.6
Coulombic efficiency (%)	98.5	93.3
Yields [mol_product/mol_glucose]
Y _2KGA	0.90 ± 0.03	0.90 ± 0.02
Y _{acetic acid}	0.07 ± 0.01	0.14 ± 0.01
Y _{gluconic acid}	0.31 ± 0.06	0.09 ± 0.03
Y _{gluconic acid}	0.25 ± 0.03	0.09 ± 0.04
Y _electrons	3.94 ± 0.11	3.88 ± 0.07
Rates [mmol/(gCDW h)]
r _glucose	−0.81 ± 0.14	−1.10 ± 0.21
r _{acetic acid}	0.06 ± 0.01	0.16 ± 0.03
r _2KGA	0.73 ± 0.13	0.99 ± 0.19
r _{gluconic acid}	0.25 ± 0.06	0.10 ± 0.04
r _{gluconic acid}	−0.20 ± 0.04	−0.10 ± 0.05
r _electrons	3.18 ± 0.55	4.28 ± 0.82

Data are fitted with linear regression using datasets from ten ([Fe(CN)₆]^3−/4−) and four ([Co(bpy)₃]^3+/2+) biological replicates with a total of 79 and 36 samples, respectively (compare Additional file 1: Fig. S3). Carbon balance is calculated from the fitted rates considering carbon content of molecules and assuming equimolar CO₂ production when making acetate from glucose. Gluconic acid is a product in the first 100 h and serves as a substrate thereafter; hence, 2 yields and rates are given

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