Fig. 5
From: Engineering broad-spectrum digestion of polyuronides from an exolytic polysaccharide lyase
Endolytic and exolytic modes of polysaccharide cleavage. a Endolytic enzyme binds to an internal site on the polysaccharide, cleaves the O-glycosidic bond via β-elimination, and detaches, resulting in products of various sizes. b Exolytic enzyme binds to one end of the polysaccharide, undergoes β-elimination, clipping off an unsaturated monomer while simultaneous sliding down the polysaccharide to the next cleavable bond. The process repeats until the entire polysaccharide has been processed into unsaturated monomers