|
Enzyme
|
Activity [U/mg] high copy vector
|
Activity [U/mg] low copy vector
|
|---|
|
Thiolase
|
|
Sc
ERG10
|
0.043 ± 0.009
|
0.10 ± 0.005
|
|
Ca
thlA
|
0.15 ± 0.04
|
4.96 ± 0.4
|
|
None
|
0.003 ± 0.0003
|
0.004 ± 0.002
|
|
Acetyl-CoA:malonyl-CoA acyltransferase
|
|
Ss
nphT7
|
NM
|
0.12 ± 0.009
|
|
None
|
NM
|
0.002 ± 0.001
|
|
3-Hydroxybutyryl-CoA DH
|
|
Ca
hbd
|
0.31 ± 0.04
|
1.47 ± 0.19
|
|
None
|
0.011 ± 0.004
|
0.063 ± 0.01
|
|
Crotonase
|
|
Ca
crt
|
3.5 ± 1.07
|
35.64 ± 3.2
|
|
None
|
0.17 ± 0.04
|
4.09 ± 2.2
|
|
Trans-2-enoyl-CoA reductase
|
|
Eg
ter
|
ND
|
0.002 ± 0.001
|
|
Td
ter
|
ND
|
0.0103 ± 0.002
|
|
None
|
ND
|
ND
|
|
Crotonyl-CoA reductase
|
|
Sco
ccr
|
NM
|
0.001 ± 0.00009
|
|
None
|
NM
|
ND
|
|
Butyraldehyde-DH
|
|
Ca
adhE2 (pVS1)
|
0.00027 ± 0.00002
|
0.0039 ± 0.003
|
|
Ca
adhE2 + Ec
eutE (pVS6)
|
0.00091 ± 0.0004
|
0.06 ± 0.009
|
|
Ec
eutE
|
0.014 ± 0.0083
|
0.044 ± 0.004
|
|
None
|
ND
|
0.0022 ± 0.0009
|
|
Butanol-DH
|
|
Ca
adhE2 (pVS1)
|
0.07 ± 0.02
|
0.062 ± 0.015
|
|
Ca
adhE2 (pVS6)
|
0.053 ± 0.02
|
0.076 ± 0.005
|
|
Ca
bdhB
|
0.073 ± 0.03
|
0.044 ± 0.008
|
|
None
|
0.049 ± 0.01
|
0.04 ± 0.009
|
- The mean values of three independent replicates are shown with standard deviations. One unit (U) is defined as the conversion of 1 µmol of substrate into the corresponding product per 1 min. The term “none” refers to the empty vector control, which means for the high copy variants (2 µ) pRS62K and for the low copy variants (CEN6/ARS4) pRS41K. Genes from Saccharomyces cerevisiae (Sc), Clostridium acetobutylicum (Ca), E.coli (Ec), Euglena gracilis (Eg), Streptomyces collinus (Sco), Streptomyces sp. CL190 (Ss), and Treponema denticola (Td) are indicated by prefixes in superscript
-
ND not detectable, NM not measured
