Comparative analysis of taxonomic, functional, and metabolic patterns of microbiomes from 14 full-scale biogas reactors by metagenomic sequencing and radioisotopic analysis

Table 1 Operating conditions of the full-scale biogas plants and related parameters of the samples

Sample name	Plant name	Main component in the feedstock	Reactor volume (m³)	HRT (days)	Biogas production (m³/m³/d)	Operating temperature (^oC)	VFA (mM)	Ammonia-N (g/L)	pH	Free ammonia (g/L)
MM1	Nysted	Manure^a	4800	21	2.85	37	6.32 ± 1.90	2.47 ± 0.05	7.83 ± 0.05	0.46 ± 0.07
MM2	Fangel	Manure	8000	32	2.25	40	6.93 ± 0.54	4.22 ± 0.01	8.18 ± 0.03	1.42 ± 0.09
MM3	Maabjerg	Manure	37,500	24	1.16	40	5.58 ± 0.20	2.63 ± 0.05	7.75 ± 0.07	0.42 ± 0.07
MT1	Sinding	Manure	750	22	3.2	52	1.95 ± 0.44	2.53 ± 0.04	7.82 ± 0.04	0.46 ± 0.04
MT2a	Blåhoj first step	Manure	1400	11	2.36	50	21.29 ± 8.54	2.96 ± 0.05	8.18 ± 0.08	0.99 ± 0.14
MT2b	Blåhoj second step	Manure	1400	11	2.36	52	10.74 ± 2.33	3.3 ± 0.05	8.36 ± 0.05	1.44 ± 0.10
MT3a	Lemvig first step	Manure	2400	15	2.86	52	4.49 ± 0.45	2.46 ± 0.04	8.10 ± 0.04	0.73 ± 0.05
MT3b	Lemvig second step	Manure	2400	3	0.74	52	1.05 ± 0.19	2.31 ± 0.07	8.10 ± 0.10	0.69 ± 0.13
MT4	Filskov	Manure	880	11	4.04	53	15.67 ± 0.17	2.38 ± 0.05	8.03 ± 0.06	0.63 ± 0.11
SM1	Maabjerg	Sewage sludge	9000	24	0.67	37	1.19 ± 0.13	0.47 ± 0.02	7.01 ± 0.04	0.02 ± 0.01
SM2	Luntoft	Sewage sludge	5000	30	3.3	37	0.65 ± 0.03	0.92 ± 0.03	7.11 ± 0.07	0.04 ± 0.01
SM3	Avedøre	Sewage sludge	6000	25	0.5	39	0.64 ± 0.07	0.53 ± 0.06	7.1 ± 0.03	0.02 ± 0.01
SM4	Helsinger	Sewage sludge	1400	19	0.5	37	0.60 ± 0.06	1.1 ± 0.02	7.33 ± 0.05	0.07 ± 0.01
SM5	Fakse	Sewage sludge	670	22	0.36	36	1.70 ± 1.29	1.24 ± 0.06	7.53 ± 0.09	0.13 ± 0.02

^a Manure from both cattle and swine. The co-fermentation feedstocks were industrial organic wastes, which accounted for around 10 %

ISSN: 2731-3654