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Table 3 The strains and primers applied in this study

From: Systematic engineering of the central metabolism in Escherichia coli for effective production of n-butanol

  Characteristics Source
Strains
BuT-8 ΔfrdA ɸ80attB:: PλPL-crt  
  ΔadhE::ɸ80attB::PλPL-pha-hbd  
  ΔldhA::λattB::PλPL-adhE2 19
BuT-8-Fdh1 as P21attB:: Ptac-fdh1 34
BuT-9 as But-8-Fdh ∆lpdA λattB::PλPL-lpdA * PλPL-aceEF This study
BuT-10 as But-9 PλPL-zwf PλPL-UdhA This study
BuT-12 as But-10 ΔatoD::PλPL-pgl This study
BuT-13 as But-12 ∆pgi This study
BuT-14 as But-13 lacO-gltA This study
Primers
RC10178 ATAAGGATCCATATCTAACACCGTGCGTG  
RC11210 CACACCATATGTTAGAATTCATTACCTTCG  
RC11403 TTTGCGGTACCAAGCCCTTTGCAAATTGC  
RC11404 CAGCAGAGCTCGAATGGATCGCGTTATC  
RC11405 AGAATCATATGGCGGTAACGCAAACAG  
RC11406 CTTAAGGATCCTAACCCGGTACTTAAGCCAG  
RC11407 CGTAAGGTACCTGACGCATGCGCGTTTG  
RC11408 ACTTAGAGCTCTAAATGCGGCTTCCACCAG  
RC11409 GCCCTCATATGCCACATTCCTACGATTAC  
RC11410 TGTTCGGATCCATAAAAGCAACAGAATGGTAAC  
RC11417 CCAAGCCCTTTGCAAATTGC  
RC11418 CTCGAATGGATCGCGTTATC  
RC11419 CCTGACGCATGCGCGTTTG  
RC11420 CTAAATGCGGCTTCCACCAG  
RC12058 AATAACATATGTCAGAACGTTTCCCAAATG  
RC12059 CTATCTCTAGACGTTGAGTTTTCTGGAACC  
RC12060 CCAGTTCGAGGTCTTTTTTCG  
RC12085 TATGGGGTACCAGTTCGAGGTCTTTTTTCG  
RC12086 CAATGGAGCTCTGCTTCATCTGCTAAGG  
RC12154 GCGATATCGTCGGTCAACC  
RC12155 TGAGAAGCTTCAGTCCGCATCACCAGAG  
RC12171 GCAAGCTTATTTCTTCTGTCCATAAGC  
RC12215 GTCCATCGCCTATACCAAACCAGAAGTTGCATG  
RC12216 CATGCAACTTCTGGTTTGGTATAGGCGATGGAC  
RC12288 AACTGCTCGAGTTACTTCTTCTTCGCTTTCG  
RC12289 AAGTGGATCCATACCCGTCGTCTTTCAGG  
RC12290 CCATGAGCTCGGCTTTTTTCTGGTAATCTC  
RC12314 TCTGGGGATCCTTCTGAAATGAGCTGTTGAC  
RC12331 ACTCTCGAATTCTGGTCGTCCTATCGCTTC  
RC13001 TTGAATTCCGCCTTTAAAGATCGCCATG  
RC13034 CATCTCACCAGATATCATGC  
RC13035 AATCGGAGCTCGAAAGTGAACTGTTTGG  
RC13195 ATCTTCCCGGGCGGAATTCATTACCGTTC  
RC13196 GAAATTGTTATCCGCTCACAATTCCGGGTACCCAATTC  
RC13197 CAGCAAAATACCTTCATCACC  
RC13198 TTCAGGGGAAGAGAGGCTG  
RC13199 TCAATGGGCCCACACTGTTACATAAGTTAATC  
RC13200 TTAATGTCGACGATTGCTAAGTACTTGATTCG  
RC13201 GGTACCCAGAAGCCACAG  
RC13292 ATCCCGGGAAGCAAACAGTTTATATCGC  
RC13293 ATCTCGAGTTAGTGTGCGTTAACCACCAC  
RC14025 GAGGAATTCTGTAGGCTGGAGCTGCTTC  
RC14026 AACGGTCGACATGGGAATTAGCCATGG