Table 3 The strains and primers applied in this study
Characteristics | Source | |
|---|---|---|
Strains | ||
BuT-8 | ΔfrdA ɸ80attB:: PλPL-crt | |
ΔadhE::ɸ80attB::PλPL-pha-hbd | ||
ΔldhA::λattB::PλPL-adhE2 | 19 | |
BuT-8-Fdh1 | as P21attB:: Ptac-fdh1 | 34 |
BuT-9 | as But-8-Fdh ∆lpdA λattB::PλPL-lpdA * PλPL-aceEF | This study |
BuT-10 | as But-9 PλPL-zwf PλPL-UdhA | This study |
BuT-12 | as But-10 ΔatoD::PλPL-pgl | This study |
BuT-13 | as But-12 ∆pgi | This study |
BuT-14 | as But-13 lacO-gltA | This study |
Primers | ||
RC10178 | ATAAGGATCCATATCTAACACCGTGCGTG | |
RC11210 | CACACCATATGTTAGAATTCATTACCTTCG | |
RC11403 | TTTGCGGTACCAAGCCCTTTGCAAATTGC | |
RC11404 | CAGCAGAGCTCGAATGGATCGCGTTATC | |
RC11405 | AGAATCATATGGCGGTAACGCAAACAG | |
RC11406 | CTTAAGGATCCTAACCCGGTACTTAAGCCAG | |
RC11407 | CGTAAGGTACCTGACGCATGCGCGTTTG | |
RC11408 | ACTTAGAGCTCTAAATGCGGCTTCCACCAG | |
RC11409 | GCCCTCATATGCCACATTCCTACGATTAC | |
RC11410 | TGTTCGGATCCATAAAAGCAACAGAATGGTAAC | |
RC11417 | CCAAGCCCTTTGCAAATTGC | |
RC11418 | CTCGAATGGATCGCGTTATC | |
RC11419 | CCTGACGCATGCGCGTTTG | |
RC11420 | CTAAATGCGGCTTCCACCAG | |
RC12058 | AATAACATATGTCAGAACGTTTCCCAAATG | |
RC12059 | CTATCTCTAGACGTTGAGTTTTCTGGAACC | |
RC12060 | CCAGTTCGAGGTCTTTTTTCG | |
RC12085 | TATGGGGTACCAGTTCGAGGTCTTTTTTCG | |
RC12086 | CAATGGAGCTCTGCTTCATCTGCTAAGG | |
RC12154 | GCGATATCGTCGGTCAACC | |
RC12155 | TGAGAAGCTTCAGTCCGCATCACCAGAG | |
RC12171 | GCAAGCTTATTTCTTCTGTCCATAAGC | |
RC12215 | GTCCATCGCCTATACCAAACCAGAAGTTGCATG | |
RC12216 | CATGCAACTTCTGGTTTGGTATAGGCGATGGAC | |
RC12288 | AACTGCTCGAGTTACTTCTTCTTCGCTTTCG | |
RC12289 | AAGTGGATCCATACCCGTCGTCTTTCAGG | |
RC12290 | CCATGAGCTCGGCTTTTTTCTGGTAATCTC | |
RC12314 | TCTGGGGATCCTTCTGAAATGAGCTGTTGAC | |
RC12331 | ACTCTCGAATTCTGGTCGTCCTATCGCTTC | |
RC13001 | TTGAATTCCGCCTTTAAAGATCGCCATG | |
RC13034 | CATCTCACCAGATATCATGC | |
RC13035 | AATCGGAGCTCGAAAGTGAACTGTTTGG | |
RC13195 | ATCTTCCCGGGCGGAATTCATTACCGTTC | |
RC13196 | GAAATTGTTATCCGCTCACAATTCCGGGTACCCAATTC | |
RC13197 | CAGCAAAATACCTTCATCACC | |
RC13198 | TTCAGGGGAAGAGAGGCTG | |
RC13199 | TCAATGGGCCCACACTGTTACATAAGTTAATC | |
RC13200 | TTAATGTCGACGATTGCTAAGTACTTGATTCG | |
RC13201 | GGTACCCAGAAGCCACAG | |
RC13292 | ATCCCGGGAAGCAAACAGTTTATATCGC | |
RC13293 | ATCTCGAGTTAGTGTGCGTTAACCACCAC | |
RC14025 | GAGGAATTCTGTAGGCTGGAGCTGCTTC | |
RC14026 | AACGGTCGACATGGGAATTAGCCATGG | |