|
Strains
|
|
BuT-8
|
ΔfrdA ɸ80attB:: PλPL-crt
| |
| |
ΔadhE::ɸ80attB::PλPL-pha-hbd
| |
| |
ΔldhA::λattB::PλPL-adhE2
|
19
|
|
BuT-8-Fdh1
|
as P21attB:: Ptac-fdh1
|
34
|
|
BuT-9
|
as But-8-Fdh ∆lpdA λattB::PλPL-lpdA
* PλPL-aceEF
|
This study
|
|
BuT-10
|
as But-9 PλPL-zwf PλPL-UdhA
|
This study
|
|
BuT-12
|
as But-10 ΔatoD::PλPL-pgl
|
This study
|
|
BuT-13
|
as But-12 ∆pgi
|
This study
|
|
BuT-14
|
as But-13 lacO-gltA
|
This study
|
|
Primers
|
|
RC10178
|
ATAAGGATCCATATCTAACACCGTGCGTG
| |
|
RC11210
|
CACACCATATGTTAGAATTCATTACCTTCG
| |
|
RC11403
|
TTTGCGGTACCAAGCCCTTTGCAAATTGC
| |
|
RC11404
|
CAGCAGAGCTCGAATGGATCGCGTTATC
| |
|
RC11405
|
AGAATCATATGGCGGTAACGCAAACAG
| |
|
RC11406
|
CTTAAGGATCCTAACCCGGTACTTAAGCCAG
| |
|
RC11407
|
CGTAAGGTACCTGACGCATGCGCGTTTG
| |
|
RC11408
|
ACTTAGAGCTCTAAATGCGGCTTCCACCAG
| |
|
RC11409
|
GCCCTCATATGCCACATTCCTACGATTAC
| |
|
RC11410
|
TGTTCGGATCCATAAAAGCAACAGAATGGTAAC
| |
|
RC11417
|
CCAAGCCCTTTGCAAATTGC
| |
|
RC11418
|
CTCGAATGGATCGCGTTATC
| |
|
RC11419
|
CCTGACGCATGCGCGTTTG
| |
|
RC11420
|
CTAAATGCGGCTTCCACCAG
| |
|
RC12058
|
AATAACATATGTCAGAACGTTTCCCAAATG
| |
|
RC12059
|
CTATCTCTAGACGTTGAGTTTTCTGGAACC
| |
|
RC12060
|
CCAGTTCGAGGTCTTTTTTCG
| |
|
RC12085
|
TATGGGGTACCAGTTCGAGGTCTTTTTTCG
| |
|
RC12086
|
CAATGGAGCTCTGCTTCATCTGCTAAGG
| |
|
RC12154
|
GCGATATCGTCGGTCAACC
| |
|
RC12155
|
TGAGAAGCTTCAGTCCGCATCACCAGAG
| |
|
RC12171
|
GCAAGCTTATTTCTTCTGTCCATAAGC
| |
|
RC12215
|
GTCCATCGCCTATACCAAACCAGAAGTTGCATG
| |
|
RC12216
|
CATGCAACTTCTGGTTTGGTATAGGCGATGGAC
| |
|
RC12288
|
AACTGCTCGAGTTACTTCTTCTTCGCTTTCG
| |
|
RC12289
|
AAGTGGATCCATACCCGTCGTCTTTCAGG
| |
|
RC12290
|
CCATGAGCTCGGCTTTTTTCTGGTAATCTC
| |
|
RC12314
|
TCTGGGGATCCTTCTGAAATGAGCTGTTGAC
| |
|
RC12331
|
ACTCTCGAATTCTGGTCGTCCTATCGCTTC
| |
|
RC13001
|
TTGAATTCCGCCTTTAAAGATCGCCATG
| |
|
RC13034
|
CATCTCACCAGATATCATGC
| |
|
RC13035
|
AATCGGAGCTCGAAAGTGAACTGTTTGG
| |
|
RC13195
|
ATCTTCCCGGGCGGAATTCATTACCGTTC
| |
|
RC13196
|
GAAATTGTTATCCGCTCACAATTCCGGGTACCCAATTC
| |
|
RC13197
|
CAGCAAAATACCTTCATCACC
| |
|
RC13198
|
TTCAGGGGAAGAGAGGCTG
| |
|
RC13199
|
TCAATGGGCCCACACTGTTACATAAGTTAATC
| |
|
RC13200
|
TTAATGTCGACGATTGCTAAGTACTTGATTCG
| |
|
RC13201
|
GGTACCCAGAAGCCACAG
| |
|
RC13292
|
ATCCCGGGAAGCAAACAGTTTATATCGC
| |
|
RC13293
|
ATCTCGAGTTAGTGTGCGTTAACCACCAC
| |
|
RC14025
|
GAGGAATTCTGTAGGCTGGAGCTGCTTC
| |
|
RC14026
|
AACGGTCGACATGGGAATTAGCCATGG
| |
