Fig. 1

Molecular and phylogenetic characterization of the Chlamydomonas std1 mutant. a In std1, the paromomycin-resistance cassette (AphVIII gene, white box) is inserted within the third exon of a gene annotated as a DYRK kinase (Cre07.g337300, v5.5). The gene structure was deduced from three overlapping RT-PCR. b STD1 transcript levels were analyzed by RT-PCR in WT, std1 and two complemented lines (std1::STD1-1 and std1::STD1-2) by amplifying a 1421 bp product. A 456 bp RT-PCR actin product was amplified as a loading control. Sequence primers for RT-PCR are given in Additional file 1: Table S2. c The level of STD1 protein was analyzed by immunodetection in WT, std1 and two complemented lines (std1::STD1-1 and std1::STD1-2). d Phylogenetic analysis of the DYRK protein family indicates that STD1 (referred to here as DYRKP) belongs to a new DYRK subfamily specific to plants