Key enzymes catalyzing glycerol to 1,3-propanediol

Table 1 The codings of GDHt and DDH from different organisms

Organism	Gene name			Gene length			Protein molecular mass (Da)
Organism	α	β	γ	α	β	γ	α	β	γ
K. pneumoniae	gldA	gldB	gldC	1668	585	426	60,621	21,310	16,094
K. oxytoca	pddA	pddB	pddC	1668	675	522	60,348	24,113	19,173
K. pneumoniae XJPD-Li	dhaB	dhaC	dhaE	1668	584	425	60,702	21,322	16,101
C. pasteurianum	dhaB	dhaC	dhaE	1665	540	441	60,813	19,549	16,722
C. freundii	dhaB	dhaC	dhaE	1665	540	441	60,813	19,549	16,722
C. freundii	dhaB	dhaC	dhaE	1668	585	429	60,433	21,487	16,121
Clostridium butyrium	dhaB1			2364			88,074
S.typhimurium	pduC	pduD	pduE	1665	675	522	60,307	24,157	19,131

The dhaBCE genes of K. pneumoniae XJPD-Li, C.pasteurianum and C.freundii; the gldABC genes of K.pneumoniae encode coenzyme B₁₂-dependent glycerol dehydratase; The pddABC genes of K.oxytoca and the pduCDE genes of S.typhimurium encode coenzyme B₁₂-dependent diol dehydatase; The dhaB1 gene of Clostridium butyrium encode coenzyme B₁₂-independent glycerol dehydratase; α, large subunit; β, intermediate subunit; γ, small subunit

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