Table 6 Enzymatic properties of GDH from various organisms
Enzymatic properties | GDH from K. pneumoniae DSM 2026 | GDH from E.coli BL21(DE3)/pET-gldA | GDH from E.coli BL21(DE3)/pET-gldA-74 |
|---|---|---|---|
Optimal pH | 11.0 | 11.0 | 12.5 |
Optimal temperature (°C) | 50 | 65 | 55 |
Km (glycerol) (mmol/L) | 0.79 | 0.58 | 0.63 |
Km (NAD) (mmol/L) | 0.13 | 0.74 | 0.77 |