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Table 6 Enzymatic properties of GDH from various organisms

From: Key enzymes catalyzing glycerol to 1,3-propanediol

Enzymatic properties GDH from K. pneumoniae DSM 2026 GDH from E.coli BL21(DE3)/pET-gldA GDH from E.coli BL21(DE3)/pET-gldA-74
Optimal pH 11.0 11.0 12.5
Optimal temperature (°C) 50 65 55
Km (glycerol) (mmol/L) 0.79 0.58 0.63
Km (NAD) (mmol/L) 0.13 0.74 0.77