Fig. 5

The impact of CclEXL1 on catalytic hydrolysis of microcrystalline cellulose by C. clariflavum cellulosome fractions. Hydrolysis of microcrystalline cellulose (Avicel) was performed by the cellulosome fractions of C. clariflavum (25 µg/mL) with (full symbols) or without (empty symbols) the addition of CclEXL1 (0.5 µM). Samples were taken at 24, 48, and 72 h, and the amount of the released reducing sugars was assessed. Hydrolysis was performed by the high-molecular-weight fraction, MCCI (a), the low-molecular-weight fraction, MCCII (b), and a combination of MCCI and MCCII (to a combined concentration of 25 µg/mL) (c). Standard deviations are indicated, where two asterisks indicate p < 0.01 and three asterisks indicate p < 0.001 (two-tailed t test)