Penicillium echinulatum secretome analysis reveals the fungi potential for degradation of lignocellulosic biomass

Table 3 Identified hemicellulose-degradation enzymes and spectrum count in different carbon sources by wild-type 2HH and mutant S1M29 of Penicillium echinulatum at 96 h during submerged cultivation

Accession number	Identified proteins	CAZyme	Organism	MW (kDa)	Secretion^b	Spectrum count^a
						2HH				S1M29
						Glu	Gly	SCB	Cel	Glu	Gly	SCB	Cel
g2645	Xylanase	GH10 (CBM1)	Penicillium decumbens	44	Y	0	0	1	10	0	0	21	27
g1025	Endo-β-1,4-xylanase	GH10	Penicillium oxalicum	40	Y	0	0	12	21	0	0	0	1
g3831	Endo-β-1,4-xylanase	GH30	Penicillium oxalicum	53	Y	0	0	5	11	0	0	0	2
g5166	Endo-β-1,4-xylanase	GH11 (CBM1)	Penicillium oxalicum	31	Y	0	0	0	2	1	0	2	5
g3240	β-xylosidase	GH43	Penicillium oxalicum	64	Y	0	0	0	8	0	0	0	0
g1531	β-xylosidase	GH43	Penicillium oxalicum	38	Y	0	0	8	0	0	0	0	0
g2200	β-xylosidase	GH3	Penicillium oxalicum	85	Y	0	0	0	1	0	0	0	15
g5735	α-mannosidase	GH47	Penicillium oxalicum	56	Y	0	14	7	3	0	16	0	3
g395	Exo-α-1,6-mannosidase	GH125	Sphaerulina musiva	56	N	0	12	0	2	0	7	0	0
g5829	β-1,4-mannanase	GH5 (CBM1)	Penicillium oxalicum	47	Y	0	0	7	8	0	2	0	12
g8513	Sorbitol/xylulose reductase	GH99	Aspergillus clavatus	28	N	0	13	6	22	0	2	1	1
g315	Acetyl xylan esterase	CE1 (CBM1)	Penicillium oxalicum	41	Y	0	0	0	0	0	0	1	1
g2707	Acetyl xylan esterase	CE5	Penicillium oxalicum	24	Y	0	0	0	5	0	0	1	2

^aSecretomic analysis based on spectral counting. A quantitative analysis was conducted for samples grown on cellulose or SCB (mean of triplicates), while a semiquantitative analysis for samples grown on glucose or glycerol (one replicate) was performed. The complete protein reports are given in Additional file 3
^bThe secretion of each protein was verified by the softwares SignalP, SecretomeP and YLoc. When at least two of three softwares give a positive result the protein was considered secreted

ISSN: 2731-3654