Novel Bacillus subtilis IND19 cell factory for the simultaneous production of carboxy methyl cellulase and protease using cow dung substrate in solid-substrate fermentation

Table 4 ANOVA for the quadratic model for CMCase activity and protease activity of B. subtilis IND19

Source	Sum of squares	df	Mean square	F value	p value
CMCase activity of B. subtilis IND19
Model	2.75E+05	9	3.05E+04	67.14	<0.0001	Significant
A-pH	5.27E+04	1	5.27E+04	116.04	<0.0001
B-Peptone	4.46E+04	1	4.46E+04	98.04	<0.0001
C-NaH₂PO₄	3.26E+04	1	3.26E+04	71.81	<0.0001
AB	9.25E+03	1	9.25E+03	20.35	0.0011
AC	2.88E+02	1	2.88E+02	0.63	0.4445
BC	924	1	924	2.03	0.1843
A ²	69,800.17	1	69,800.17	153.57	<0.0001
B ²	1.89E+04	1	1.89E+04	41.51	<0.0001
C ²	6.87E+04	1	6.87E+04	151.24	<0.0001
Residual	4.55E+03	10	454.51
Lack of fit	3456.22	5	691.24	3.17		Not significant
Pure error	1.09E+03	5	217.77
Cor total	2.79E+05	19
Protease activity of B. subtilis IND19
Model	2.95E+09	9	3.27E+06	197.54	<0.0001	Significant
A-pH	2.30E+04	1	2.30E+04	1.39	0.266
B-Peptone	4.82E+0.005	1	4.82E+0.005	291.22	<0.0001
C-NaH₂PO₄	7.02E+05	1	7.02E+05	42.35	<0.0001
AB	2.412E+0.05	1	2.412E+0.05	14.55	0.0034
AC	2.12E+06	1	2.12E+06	127.58	<0.0001
BC	8.70E+06	1	8.70E+06	524.67	<0.0001
A ²	1.11E+07	1	1.11E+07	670.76	<0.0001
B ²	2.31E+06	1	2.31E+06	139.47	<0.0001
C ²	1.43E+04	1	1.43E+04	0.86	0.3744
Residual	1.66E+05	10	16,575.16
Lack of fit	23,264.15	5	4652.83	0.16	0.9658	Not significant
Pure error	1.43E+05	5	28,497.5
Cor total	2.96E+07	19

ISSN: 2731-3654