Skip to main content

Table 4 ANOVA for the quadratic model for CMCase activity and protease activity of B. subtilis IND19

From: Novel Bacillus subtilis IND19 cell factory for the simultaneous production of carboxy methyl cellulase and protease using cow dung substrate in solid-substrate fermentation

Source Sum of squares df Mean square F value p value  
CMCase activity of B. subtilis IND19
 Model 2.75E+05 9 3.05E+04 67.14 <0.0001 Significant
 A-pH 5.27E+04 1 5.27E+04 116.04 <0.0001  
 B-Peptone 4.46E+04 1 4.46E+04 98.04 <0.0001  
 C-NaH2PO4 3.26E+04 1 3.26E+04 71.81 <0.0001  
 AB 9.25E+03 1 9.25E+03 20.35 0.0011  
 AC 2.88E+02 1 2.88E+02 0.63 0.4445  
 BC 924 1 924 2.03 0.1843  
 A 2 69,800.17 1 69,800.17 153.57 <0.0001  
 B 2 1.89E+04 1 1.89E+04 41.51 <0.0001  
 C 2 6.87E+04 1 6.87E+04 151.24 <0.0001  
 Residual 4.55E+03 10 454.51    
 Lack of fit 3456.22 5 691.24 3.17   Not significant
 Pure error 1.09E+03 5 217.77    
 Cor total 2.79E+05 19     
Protease activity of B. subtilis IND19
 Model 2.95E+09 9 3.27E+06 197.54 <0.0001 Significant
 A-pH 2.30E+04 1 2.30E+04 1.39 0.266  
 B-Peptone 4.82E+0.005 1 4.82E+0.005 291.22 <0.0001  
 C-NaH2PO4 7.02E+05 1 7.02E+05 42.35 <0.0001  
 AB 2.412E+0.05 1 2.412E+0.05 14.55 0.0034  
 AC 2.12E+06 1 2.12E+06 127.58 <0.0001  
 BC 8.70E+06 1 8.70E+06 524.67 <0.0001  
 A 2 1.11E+07 1 1.11E+07 670.76 <0.0001  
 B 2 2.31E+06 1 2.31E+06 139.47 <0.0001  
 C 2 1.43E+04 1 1.43E+04 0.86 0.3744  
 Residual 1.66E+05 10 16,575.16    
 Lack of fit 23,264.15 5 4652.83 0.16 0.9658 Not significant
 Pure error 1.43E+05 5 28,497.5    
 Cor total 2.96E+07 19